1887

Abstract

SUMMARY

The ‘b’ protein components specific to virus infected tobacco leaves (Gianinazzi, Martin & Vallée, 1970) can be partially purified by preferential extraction at pH 2.8. Evidence is presented that they are rich in aromatic amino acids. Results of treatment of the proteins with SDS and subsequent separation by gel electrophoresis in the presence of SDS suggest that b, b and b are composed of the same monomer of mol. wt. about 16000 whilst b is composed of a monomer of mol. wt. about 29000. By purifying and concentrating the soluble protein extracts of water inoculated leaves, further evidence is provided that the ‘b’ proteins are not normal constituents of healthy tobacco leaves.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-34-2-345
1977-02-01
2022-08-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/34/2/JV0340020345.html?itemId=/content/journal/jgv/10.1099/0022-1317-34-2-345&mimeType=html&fmt=ahah

References

  1. Barker H. 1975; Effects of virus infection and polyacrylic acid on leaf proteins. Journal of General Virology 28:155–158
    [Google Scholar]
  2. Colby C., Morgan M. J. 1971; Interferon induction and action. Annual Review of Microbiology 25:333–360
    [Google Scholar]
  3. Davis B. J. 1964; Disc electrophoresis. II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 2:404–427
    [Google Scholar]
  4. De Clercq E., Eckstein F., Merigan T. C. 1970; Structural requirements for synthetic polyanions to act as interferon inducers. Annals of the New York Academy of Sciences 173:444–461
    [Google Scholar]
  5. Galabov A. S., Galabov S. M. 1973; Interferon induction by detoxicated bacterial endotoxins. Acta Virologica 17:493–500
    [Google Scholar]
  6. Gianinazzi S. 1970; Hypersensibilité au virus de la mosaïque du tabac chez divers Nicotiana. Effet thermique et proteins solubles Thèse de Docteurès-sciences University of Geneva; Switzerland:
    [Google Scholar]
  7. Gianinazzi S., Kassanis B. 1974; Virus resistance induced in plants by polyacrylic acid. Journal of General Virology 23:1–9
    [Google Scholar]
  8. Gianinazzi S., Martin C. 1975; A naturally occurring active factor inducing resistance to virus infection in plants. Phytopathologische Zeitschrift 83:23–26
    [Google Scholar]
  9. Gianinazzi S., Martin C., Vallée J. C. 1970; Hypersensibilité aux virus, température et proteines solubles chez le Nicotiana Xanthi n.c. Apparition de nouvelles macromolécules lors de la repression de la synthèse virale. Compte rendu de l’Académie des sciences de Paris D 270:2383–2386
    [Google Scholar]
  10. Gianinazzi S., Vallée J. C., Martin C. 1969; Hypersensibilité aux virus, température et protéines solubles chez le Nicotiana Xanthi n.c. Compte rendu de l’Académie des Sciences de Paris D 268:800–802
    [Google Scholar]
  11. Kassanis B., Gianinazzi S., White R. F. 1974; A possible explanation of the resistance of virus-infected tobacco plants to second infection. Journal of General Virology 23:11–16
    [Google Scholar]
  12. Koenig R., Stegemann H., Francksen H., Paul H. L. 1970; Protein subunits in the potato virus X group. Determination of the molecular weights by polyacrylamide electrophoresis. Biochimica et Biophysica Acta 207:184–189
    [Google Scholar]
  13. Layne E. 1957; Spectrophotometric and turbidimetric methods for measuring proteins. Methods in Enzymology 111:447–454
    [Google Scholar]
  14. Ross A. F. 1961; Localized acquired resistance to plant virus infection in hypersensitive hosts. Virology 14:329–339
    [Google Scholar]
  15. van Loon L. C. 1975; Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. ‘Samsun’ and ‘Samsun NN’. IV. Similarity of qualitative changes of specific proteins after infection with different viruses and their relationship to acquired resistance. Virology 67:566–575
    [Google Scholar]
  16. van Loon L. C. 1976; Specific soluble leaf proteins in virus-infected tobacco plants are not normal constituents. Journal of General Virology 30:375–379
    [Google Scholar]
  17. van Loon L. C., Van Kammen A. 1970; Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. ‘Samsun’ and ‘Samsun NN’. II. Changes in protein constitution after infection with tobacco mosaic virus. Virology 40:199–211
    [Google Scholar]
  18. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecylsulphate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-34-2-345
Loading
/content/journal/jgv/10.1099/0022-1317-34-2-345
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error