1887

Abstract

SUMMARY

The disruption of envelopes and the fragmentation of capsids of equine herpesvirus type 1 observed in negatively stained samples were attributed to viral dehydration on carbon films during preparation for electron microscopy. Prior fixation of virus with OsO or glutaraldehyde and subsequent application of negative stain before drying minimized envelope disruption and virtually eliminated the occurrence of capsomere sheets and broken capsids. This simple procedure significantly improves electron microscopic evaluation of herpesvirus samples.

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1976-05-01
2024-04-26
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References

  1. ABODEELY R. A., LAWSON L. A., RANDALL C. C. 1970; Morphology and entry of enveloped and deenveloped equine abortion (herpes) virus. Journal of Virology 5:513–523
    [Google Scholar]
  2. BERNHARD W. 1969; A new staining procedure for electron microscopical cytology. Journal of Ultrastructure Research 27:250–265
    [Google Scholar]
  3. BOCCIARELLI D. S., ORFEI Z., MONDINI G., PERSECHINO A. 1966; The core of a bovine herpes virus. Virology 30:58–61
    [Google Scholar]
  4. BRENNER S., HORNE R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochimica et Biophysica Acta 34:103–110
    [Google Scholar]
  5. BUCKMIRE F. L. A., MURRAY R. G. E. 1970; Studies on the cell wall of Spirillum serpens. I. Isolation and partial purification of the outermost cell wall layer. Canadian Journal of Microbiology 16:1011–1022
    [Google Scholar]
  6. COHEN A. L., GARNER G. E., LUND J. 1966; Zirconium and hafnium compounds as electron strains. Journal of Applied Physics 37:3921–3922
    [Google Scholar]
  7. FURLONG D., SWIFT H., ROIZMAN B. 1972; Arrangement of herpesvirus deoxyribonucleic acid in the core. Journal of Virology 10:1071–1074
    [Google Scholar]
  8. LAWRENCE W. C. 1976; Purification of equine herpes virus type I. Journal of General Virology 31:81–91
    [Google Scholar]
  9. LUDWIG H., BISWAL N., BRYANS J. T., MCCOMBS R. M. 1971; Some properties of the DNA from a new equine herpesvirus. Virology 45:534–537
    [Google Scholar]
  10. MCCOMBS R. M., BENYESH-MELNICK M., BRUNSCHWIG J. P. 1966; Biophysical studies of vesicular stomatitis virus. Journal of Bacteriology 91:803–812
    [Google Scholar]
  11. MILLONIG G. 1961; Advantages of a phosphate buffer for OsO4 solutions in fixation. Journal of Applied Physics 32:1637
    [Google Scholar]
  12. PERDUE M. L., KEMP M. C, RANDALL C. C., O’CALLAGHAN D. J. 1974; Studies of the molecular anatomy of the L-M cell strain of equine herpes virus type 1: Proteins of the nucleocapsid and intact virion. Virology 59:201–216
    [Google Scholar]
  13. SCHAFFER P. A., BRUNSCHWIG J. P., MCCOMBS R. M., BENYESH-MELNICK M. 1974; Electron microscopic studies of temperature-sensitive mutants of herpes simplex virus type 1. Virology 62:444–457
    [Google Scholar]
  14. SHARP D. G., ECKERT E. A., BEARD D., BEARD J. W. 1952; Morphology of the virus of avian erythromyelo-blastic leucosis and a comparison with the agent of Newcastle disease. Journal of Bacteriology 63:151–161
    [Google Scholar]
  15. SMITH K. O. 1963; Physical and biological observations on herpesvirus. Journal of Bacteriology 86:999–1009
    [Google Scholar]
  16. SMITH K. O. 1964; Relationship between the envelope and the infectivity of herpes simplex virus. Proceedings of the Society for Experimental Biology and Medicine 115:814–816
    [Google Scholar]
  17. SMITH K. O., BENYESH-MELNICK M., FERNBACH D. J. 1964; Studies on human leukemia. II. Structure and quantitation of myxovirus-like particles associated with human leukemia. Journal of the National Cancer Institute 33:557–570
    [Google Scholar]
  18. SMITH K. O., MELNICK J. L. 1962a; A method for staining virus particles and identifying their nucleic acid type in the electron microscope. Virology 17:480–490
    [Google Scholar]
  19. SMITH K. O., MELNICK J. L. 1962b; Recognition and quantitation of herpesvirus particles in human vesicular lesions. Science, New York 137:543–544
    [Google Scholar]
  20. SPRING S. B., ROIZMAN R. 1967; Herpes simplex virus products in productive and abortive infection. I. Stabilization with formaldehyde and preliminary analyses by isopycnic centrifugation in CsCl. Journal of Virology 1:294–301
    [Google Scholar]
  21. SYDISKIS R. J. 1969; Precursor products found in formaldehyde-fixed lysates of BHK-21 cells infected with Pseudorabies virus. Journal of Virology 4:283–291
    [Google Scholar]
  22. VERNON S. K., LAWRENCE W. C, COHEN G. C. 1974; Morphological components of herpes virus. I. Intercapsomeric fibrils and the geometry of the capsid. Intervirology 4:237–248
    [Google Scholar]
  23. WATSON D. H., RUSSELL W. C, WILDY P. 1963; Electron microscopic particle counts on herpes virus using the phosphotungstate negative staining technique. Virology 19:250–260
    [Google Scholar]
  24. WATSON D. H., WILDY P. 1963; Some serological properties of herpes virus particles studied with the electron microscope. Virology 21:100–111
    [Google Scholar]
  25. WILDY P., RUSSELL W. C., HORNE R. W. 1960; The morphology of herpes virus. Virology 12:204–222
    [Google Scholar]
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