1887

Abstract

SUMMARY

Deoxyribonucleoside triphosphate pools were analysed in both exponentially growing and serum starved wild type BHK C13 cells and in a derivative of this cell line which lacks both thymidine kinase and deoxycytidine kinase activities, before and after infection with herpes simplex virus. Serum starved BHK cells had low levels of all four deoxyribonucleoside triphosphates. In exponentially growing cells all pools were expanded, the pool of dCTP being largest and dGTP the smallest. The dATP and dTTP pools were of intermediate sizes. In exponentially growing deoxypyrimidine kinase free cells the pools, with respect to level and distribution, were the same as those observed in wild type cells. After infection with herpes simplex virus there were marked changes in the levels of all deoxyribonucleoside triphosphate pools; the most predominant being a 25- to 50-fold expansion of the dTTP pool. The pools of dCTP and dGTP also increased while the pool of dATP was very much reduced. These effects could be observed in both wild type and mutant cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-31-1-101
1976-04-01
2022-06-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/31/1/JV0310010101.html?itemId=/content/journal/jgv/10.1099/0022-1317-31-1-101&mimeType=html&fmt=ahah

References

  1. BJURSELL G., REICHARD P. 1973; Effects of thymidine on deoxyribonucleoside triphosphate pools and DNA synthesis in hamster ovary cells. Journal of Biological Chemistry 248:3904–3909
    [Google Scholar]
  2. BRAY G., BRENT P. T. 1972; Deoxyribonucleoside 5′ triphosphate pool fluctuations during the mammalian cell cycle. Biochimica et Biophysica Acta 269:184–191
    [Google Scholar]
  3. BURK R. R. 1966; An inhibition of growth detected in BHK 21/13 cells but not in a PyY transformed derivative. Nature, London 210:1080–1081
    [Google Scholar]
  4. BURTON K. 1956; The conditions and mechanism of diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemistry Journal 62:315–323
    [Google Scholar]
  5. CHAN T., MEUTH M., GREEN H. 1974; Pyrimidine excretion by cultured fibroblasts. Journal of Cellular Physiology 83:263–266
    [Google Scholar]
  6. COHEN G. H. 1972; Ribonucleotide reductase activity of synchronized KB cells infected with herpes simplex virus. Journal of Virology 9:408–418
    [Google Scholar]
  7. FOGH J., FOGH H. A. 1964; A method for direct demonstration of pleuropneumonia-like organisms in cultured cells. Proceedings of the Society for Experimental Biology and Medicine 117:899–901
    [Google Scholar]
  8. HAY J., PERERA P. A. J., MORRISON J. M., GENTRY G. A., SUBAK-SHARPE J. H. 1971 Herpes virus-specified proteins. Strategy of the Viral Genome Ciba Foundation Symposium;355–371 Edited by Wolstenholme G. E. W., O’Connor Maeve. Churchill Livingston:
    [Google Scholar]
  9. JAMIESON A. T., GENTRY G. A., SUBAK-SHARPE J. 1974; Induction of both thymidine and deoxycytidine kinase activity in herpes virus. Journal of General Virology 24:465–480
    [Google Scholar]
  10. JAMIESON A. T., SUBAK-SHARPE J. 1974; Biochemical studies on the herpes simplex virus-specified deoxypyrimidine kinase activity. Journal of General Virology 24:481–492
    [Google Scholar]
  11. KAPLAN A. S. 1969 Herpes simplex and Pseudorabies virus. Virology Monographs No. 5 New York: Springer-Verlag;
    [Google Scholar]
  12. KEIR H. M. 1968; Virus induced enzymes in mammalian cells infected with DNA viruses. Molecular Virology of VirusesXVIII, Symposium of the Society for General Microbiology67–99 Cambridge: Cambridge University Press;
    [Google Scholar]
  13. KEIR H. M., GOLD E. 1963; DNA nucleotidyl transferase and DNase from cultured cells infected with HSV. Biochimica et Biophysica Acta 72:263–276
    [Google Scholar]
  14. KEIR H. M., SUBAK-SHARPE J., SHEDDEN W. I. H., WATSON D. H., WILDY P. 1966; Immunological evidence for a specific DNA polymerase produced after infection by herpes simplex virus. Virology 30:154–162
    [Google Scholar]
  15. KIT S., DUBBS D. R., PIERASKI L. J., HEV T. C. 1962; Deletion of thymidine kinase activity from L Cells resistant to BVdR. Experimental Cell Research 31:297–312
    [Google Scholar]
  16. KOERNER J. F. 1970; Enzymes of nucleic acid metabolism. Annual Review of Biochemistry 39:219–322
    [Google Scholar]
  17. LINDBERG U., SKOOG L. 1970; A method for the determination of dATP and dTTP in picomole amounts. Analytical Biochemistry 34:152–160
    [Google Scholar]
  18. LOWRY O., ROSEBROUGH N., FARR A., RANDALL R. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  19. MACPHERSON I., STOKER M. 1962; Polyoma transformation of hamster clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  20. NEWTON A. A. 1964; Acid nucleic e lava fugione biologica 109, Institute Lambardo Academica Scienze e Convegno Antonio Basselli.
  21. NORDENSKJOLD B. A., SKOOG L., BROWN N., REICHARD P. 1970; Deoxyribonucleotide pools and deoxyribonucleic acid synthesis in cultured mouse embryo cells. Journal of Biological Chemistry 245:5360–5368
    [Google Scholar]
  22. RUSSELL W. C. 1962; A sensitive plaque assay for herpes virus. Nature, London 195:1028–1029
    [Google Scholar]
  23. SKOOG L. 1970; An enzymatic method for the determination of dCTP and dGTP in picomole amounts. European Journal of Biochemistry 17:202–208
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-31-1-101
Loading
/content/journal/jgv/10.1099/0022-1317-31-1-101
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error