Tobacco plants grown for 12 h at 25 °C in light of 10800 lux alternating with 12 h at 20 °C in darkness were reliable sources of mesophyll protoplasts, which were very susceptible to infection with tobacco rattle virus. Increasing the photoperiod or the light intensity decreased susceptibility to infection, and increasing or decreasing the photoperiod increased the fragility of the protoplasts. Ease of infection with raspberry ringspot virus seemed to parallel that with tobacco rattle virus. The optimum defined environment gave protoplasts that were at least as suitable for infection experiments as the best from glasshouse grown plants.


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