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Sera from rabbits that had been immunized with soluble antigens from rabbit-pox or vaccinia viruses showed high neutralizing activity against the intracellular form of rabbitpox virus, but little neutralizing activity against the extracellular form. They were almost ineffective in preventing the distant dissemination of rabbitpox virus through infected HeLa cell cultures. These results account for the disappointingly incomplete protection that was previously observed in rabbits immunized with vaccinia virus soluble antigens.
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