1887

Abstract

Summary

Adsorption of T phages or their ghosts to resulted in the following events. () Addition of either phage or ghost caused rapid enhancement of potassium efflux and a corresponding decrease in the intracellular concentration of potassium. () Within the first 2 min after infection, the intracellular concentration of potassium started to recover in phage, but not in ghost-infected cells. The recovery, which corresponded presumably to some repair process, was completed 4 to 5 min after infections. () The K influx was enhanced slightly, and reduced 10-fold, in the phage and in the ghost-infected cells, respectively. The repair process in the phage-infected cells was inhibited by pre-treatment of the bacteria with chloramphenicol, or by lowering the temperature from 37 to 20 °C. Formalinized phages affect the bacteria in the same manner as the ghosts. Damage which resulted from ghost adsorption, and was characterized by K transport and infective centres experiments, could be repaired to some degree by infective phage adsorption. The effects due to ghost adsorption could be partially reduced by resuspending the treated bacteria in a medium which contained a concentrate of the non-dialysable metabolites that leaked out of the ghost-treated cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-23-2-159
1974-05-01
2019-11-21
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-23-2-159
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error