1887

Abstract

SUMMARY

The molecular size of poly I.poly C required for lysis of interferon-treated L cells was the same as the size required for induction of interferon in these cells. The size requirement for both interferon induction and toxicity for interferon-treated L cells resided in the poly I strand, while variations of the poly C strand size were without effect on interferon induction or cell lysis.

The size of poly I.poly C required to induce interferon in primed L cells or primed primary rabbit kidney cells was the same as the size required to induce interferon in normal L cells or normal rabbit kidney cells. Again, these requirements proved more stringent for the poly I strand than for the poly C strand. These data suggest that both primed and normal cells recognize the same molecular ‘trigger’ for interferon induction.

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1974-04-01
2022-06-29
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References

  1. Black D. R., Eckstein F., De Clercq E., MeriGan T. C. 1973; Studies on the toxicity and antiviral activity of various polynucleotides. Antimicrobial Agents and Chemotherapy 3:198–206
    [Google Scholar]
  2. Carter W. A., Pitha P. M., Marshall L. W., Tazawa I., Tazawa S., TS’O P. O. P. 1972; Structural requirements of the rIn.rCn complex for induction of human interferon. Journal of Molecular Biology 70:567–587
    [Google Scholar]
  3. De Clercq E. 1973; Synthetic interferon inducers. In Topics in Current Chemistry Berlin, Heidelberg and New York: Springer-Verlag; (in the press)
    [Google Scholar]
  4. De Clercq E., Stewart W. E. II, De Somer P. 1973a; Studies on the mechanism of the priming effect of interferon on interferon production by cell cultures exposed to poly(rI). poly(rC). Infection and Immunity 8:309–316
    [Google Scholar]
  5. De Clercq E., Stewart W. E. II, De Somer P. 1973b; Poly(rI) more important than poly(rC) in the interferon induction process by poly(rI). Poly(rC). Virology 54:278–282
    [Google Scholar]
  6. Dianzani F., Cantagalli P., Cagnoni S., Rita G. 1968; Effect of DEAE-dextran on production of interferon induced by synthetic double-stranded RNA in L cell cultures. Proceedings of the Society for Experimental Biology and Medicine 128:708–710
    [Google Scholar]
  7. Falcoff R., Falcoff E., Catinot I. 1970; Interferon induction by and infectivity of ultraviolet-irradiated double and multistranded viral RNAs. Biochimica et biophysica acta 217:195–196
    [Google Scholar]
  8. Lampson G. P., Field A. K., Tytell A. A., Nemes M. M., Hilleman M. R. 1970; Relationship of molecular size of rIn: rCn (poly I: C) to induction of interferon and host resistance. Proceedings of the Society for Experimental Biology and Medicine 135:911–916
    [Google Scholar]
  9. Lampson G. P., Nemes M. M., Field A. K., Tytell A. A., Hilleman M. R. 1972; The effect of altering the size of poly(C) on the toxicity and antigenicity of poly I: C. Proceedings of the Society for Experimental Biology and Medicine 141:1068–1072
    [Google Scholar]
  10. Mohr S. J., Brown D. G., Coffey D. S. 1972; Size requirement of polyinosinic acid for DNA synthesis, viral resistance, and increased survival of leukaemic mice. Nature New Biology 240:250–252
    [Google Scholar]
  11. Niblack J. F., Mccreary M. B. 1971; Relationship of biological activities of poly I. poly C to homopolymer molecular weights. Nature New Biology 233:52–53
    [Google Scholar]
  12. Rosztoczy I. 1971; Interferon induction by polyinosinic. polycytidylic acid in interferon pretreated L cells in absence of DEAE-dextran. Acta Virologica 15:431
    [Google Scholar]
  13. Rosztoczy I., Mecs I. 1970; Enhancement of interferon synthesis by polyinosinic. polycytidylic acid in L cells pretreated with interferon. Acta virologica 14:398–400
    [Google Scholar]
  14. Stewart W. E. II, De Clercq E., Billiau A., Desmyter J., De Somer P. 1972a; Increased susceptibility of cells treated with interferon to the toxicity of polyriboinosinic. polyribocytidylic acid. Proceedings of the National Academy of Sciences of the United States of America 69:1851–1854
    [Google Scholar]
  15. Stewart W. E. II, De Clercq E., De Somer P. 1972c; Cellular alteration by interferon: a virus-free system for assaying interferon. Journal of Virology 10:896–901
    [Google Scholar]
  16. Stewart W. E. II, De Clercq E., De Somer P. 1973a; Specificity of interferon-induced enhancement of toxicity for double-stranded ribonucleic acids. Journal of General Virology 18:237–246
    [Google Scholar]
  17. Stewart W. E. II, De Clercq E., De Somer P., Berg K., Ogburn C. A., Paucker K. 1973b; Comparative antiviral and non-antiviral activities of highly purified interferon preparations. Nature, London 246:141–143
    [Google Scholar]
  18. Stewart W. E. II, Gosser I. B., Lockart R. Z. Jun 1971a; Priming: a non-antiviral function of interferon. Journal of Virology 7:792–801
    [Google Scholar]
  19. Stewart W. E. II, Gosser I. B., Lockart R. Z. Jun 1971b; Distinguishing characteristics of the interferon responses of primary and continuous mouse cell cultures. Journal of General Virology 13:35–50
    [Google Scholar]
  20. Stewart W. E. II, Gosser I. B., Lockart R. Z. Jun 1972b; The effect of priming with interferon on interferon production by two lines of L cells. Journal of General Virology 15:85–87
    [Google Scholar]
  21. Tytell A. A., Lampson G. P., Field A. K., Nemes M. M., Hilleman M. R. 1970; Influence of size of individual homopolynucleotides on the physical and biological properties of complexed rIn:rCn (poly I:C). Proceedings of the Society for Experimental Biology and Medicine 135:917–920
    [Google Scholar]
  22. Youngner J. S., Hallum J. V. 1969; Inhibition of induction of interferon synthesis in L cells pretreated with interferon. Virology 37:473–475
    [Google Scholar]
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