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Journal of General Virology header logo Journal of General Virology

Volume 20, Issue 1

A Comparison of Three Mycobacteriophages No Access

Abstract

SUMMARY

Mycobacteriophages B01, B02a, and B02h were studied with respect to host range, plaque morphology, electron microscopic morphology, nucleic acid composition, one-step growth characteristics, heat inactivation, adsorption, and cross-neutralization.

Phage B01 can be propagated only on . It forms plaques which are clear, circular and consist of several concentric rings. It has an elongated hexagonal head and long non-contractile tail. Its nucleic acid is double-stranded DNA with a 71% GC ratio. In heart infusion broth without Tween-80, phage B01 shows a latent period of 150 min, a rise period of 230 min, and burst size of 61.

Phage B02a is polyvalent, i.e. can be propagated on a number of different mycobacterial hosts. It forms plaques which are hazy and circular. It has a hexagonal head and long non-contractile tail. Its nucleic acid is double-stranded DNA with a 72% GC ratio. In heart infusion broth without Tween-80, phage B02a shows a latent period of 260 min, a rise period of 180 min, and a burst size of 11.

Phage B02h is also polyvalent and forms plaques that are clear and circular. This phage has a hexagonal head and a non-contractile tail. Its nucleic acid is similarly double-stranded DNA with a GC ratio of 72%. It is characterized by a latent period of 320 min, a rise period of 120 min, and a burst size of 20 in heart infusion broth without Tween-80.

The addition of 0.03% Tween-80 as recommended by Bowman (1958) decreases the burst size of all the phages studied. Tween shortens the latent period of phage B01 and B02h and the rise period of phage B01, but lengthens the latent period of phage B02a as well as the rise period of phages B02a and B02h. Heat inactivation of phage B02a is more rapid than that of B01 or B02h at 56 °C and 60 °C. Phage B01 is only slightly more heat sensitive than phage B02h.

All three phages adsorb poorly to their host bacteria. Phage B02h is strongly neutralized by anti-B02a serum, whereas neutralization of phage B02a by anti-B02h serum is considerably weaker. All three phages show some degree of cross neutralization.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1973
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1973-07-01
2025-03-19
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References

  1. Adams M. H. 1959 In Bacteriophages New York: Interscience Publishers;
     [Google Scholar]
  2. Bowman B. U. Jun 1958; Quantitative studies on some mycobacterial phage host systems. Journal of Bacteriology 76:52–62
     [Google Scholar]
  3. Bowman B. U. Jun 1969; Properties of mycobacteriophage DS6A. 1. Immunogenicity in rabbits. Proceedings of the Society of Experimental Biology and Medicine 131:196–201
     [Google Scholar]
  4. Bowman B. U. Jun, Redmond W. B. 1959; A temperate bacteriophage from Mycobacterium butyricum. American Review of Respiratory Diseases 80:232–239
     [Google Scholar]
  5. Castelnuovo G. 1970; Some properties of phage phlei; morphology, antigenic composition, protein components. In Host-Virus Relationships in Mycobacterium Nocardia and Actinomyces pp. 15–28 Edited by Juhasz S. E., Plummer G. Springfield, Illinois: C. C. Thomas Publishing Co;
     [Google Scholar]
  6. Froman S., Will D. W., Bogen E. 1954; Bacteriophage active against virulent Mycobacterium tuberculosis. I. Isolation and activity. American Journal of Public Health 44:1326–1333
     [Google Scholar]
  7. Gardner G. M., Weiser R. S. 1947; A bacteriophage for Mycobacterium smegmatis. Proceedings of the Society for Experimental Biology and Medicine 66:205–206
     [Google Scholar]
  8. Gelbart S. M., Harize M., Juhasz S. E. 1970; Phage-associated changes in lysogenic Mycobacterium smegmatis strains. In Host-Virus Relationships in Mycobacterium, Nocardia and Actinomyces pp 204–209 Edited by Juhasz S. E., Plummer G. Springfield, Illinois: C. C. Thomas Publishing Co;
     [Google Scholar]
  9. Hnatko S. I. 1952; Concentric ring formation about plaques of Mycobacterium phlei bacteriophage. Canadian Journal of Public Health 43:54–59
     [Google Scholar]
  10. Juhasz S. E., Bönicke R. 1965; Possible classification of rapidly growing mycobacteria on the basis of their phage susceptibility. Canadian Journal of Microbiology 11:235–241
     [Google Scholar]
  11. Juhasz S. E., Bönicke R. 1966; Reciprocal genetic changes in mycobacterial host-virus systems; effect of lysogeny on the phage. Nature, London 210:118–1186
     [Google Scholar]
  12. Juhasz S. E., Bönicke R. 1970; Genetic interaction in mycobacterial host-phage systems. The effect of lysogeny on the phage. Pneumonology 142:181–190
     [Google Scholar]
  13. Kozloff L. M., Sundar Raj C. V., Nagaraja R., Chapman V. A., Delong S. 1972; Structure of a transducing mycobacteriophage. Journal of Virology 9:390–393
     [Google Scholar]
  14. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
     [Google Scholar]
  15. Marmur J., Doty R. 1959; Heterogeneity in deoxyribonucleic acid. I. Dependence on composition of the configurational stability of deoxyribonucleic acids. Nature, London 183:1427–1431
     [Google Scholar]
  16. Mohelska H., Makovcova A. 1968; Quantitative studies of a mycobacterial-phage-host system. I. Characteristic properties of the phage MyF3P/59a. Zeitschrift fur allgemeine Mikrobiologie 81:29–37
     [Google Scholar]
  17. Murohashi T., Tokunaga R., Seki M. 1961; Biological properties of Y-group mycobacteriophage. Acta tuberculosea et pneumologica Scandinavica 41:33–46
     [Google Scholar]
  18. Penso G. 1955; Cycle of phage development within the bacterial cell. Protoplasma 45:251–263
     [Google Scholar]
  19. Redmond W. B. 1963; Bacteriophages of the mycobacteria. A review. Advances in Tuberculosis Research 12:191–229
     [Google Scholar]
  20. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic from its buoyant density in CsCl. Journal of Molecular Biology 4:430–443
     [Google Scholar]
  21. Sellers M. I., Baxter W. L., Runnals H. R. 1962; Growth characteristics of mycobacteriophages D28 and D29. Canadian Journal of Microbiology 8:389–399
     [Google Scholar]
  22. Sellers M. I., Runnals H. R. 1961; Mycobacteriophage. I. Physicochemical characterization. Journal of Bacteriology 81:442–447
     [Google Scholar]
  23. Wise E. M., Glickman-Rhona S. 1972; Virus satellite plaques. Nature, London 235:95–96
     [Google Scholar]
  24. Woese C. 1960; Thermal inactivation of animal viruses. Annals of the New York Academy of Sciences 83:741–751
     [Google Scholar]
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A Comparison of Three Mycobacteriophages
J. Gen. Virol. 20, 75 (1973); https://doi.org/10.1099/0022-1317-20-1-75
/content/journal/jgv/10.1099/0022-1317-20-1-75
/content/journal/jgv/10.1099/0022-1317-20-1-75
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