1887

Abstract

SUMMARY

Purified preparations of chronic bee-paralysis virus were obtained by clarifying water extracts of paralysed bees with ether and carbon tetrachloride; the virus particles were concentrated from the clarified extracts either by centrifugation or precipitation with ammonium sulphate. The preparations contained particles of three sizes, all approximately 220 Å wide and ellipsoidal in outline, but about 410, 540 or 640 Å long with sedimentation coefficients () of 97, no and 125 respectively. The shortest particles contained least nucleic acid, and preparations containing mostly short particles were less infective than those containing mostly long ones. The particles contained ribose nucleic acid with a molar base ratio of G 20 %-A 24 %-C 28 %-U 28 %. When incubated in cold acid or alkali solutions (1 ), the virus particles formed empty rounded protein shells.

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1968-03-01
2024-12-07
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References

  1. Bailey L. 1965a; The occurrence of chronic and acute bee-paralysis viruses in bees outside Britain. J. Invertebrate Path 7:167
    [Google Scholar]
  2. Bailey L. 1965b; Paralysis of the honey bee, Apis mellifera Linnaeus . J. Invertebrate Path 7:167
    [Google Scholar]
  3. Bailey L. 1967; The incidence of virus diseases in the honey bee. Ann. appl. Biol 60:43
    [Google Scholar]
  4. Bailey F., Gibbs A. J. 1964; Infection of bees with acute paralysis virus. J. Insect Path 6:395
    [Google Scholar]
  5. Bailey L., Gibbs A. J., Woods R. D. 1963; Two viruses from adult honey bees (Apis melliferaLinnaeus). Virology 21:390
    [Google Scholar]
  6. Bancroft J. B. 1962; Purification and properties of bean pod mottle virus and associated centrifuged and electrophoretic components. Virology 16:419
    [Google Scholar]
  7. Bancroft J. B., Kaesberg P. 1960; Macromolecular particles associated with alfalfa mosaic virus. Virology 19:441
    [Google Scholar]
  8. Dethe G., Notkins A. L. 1965; Ultrastructure of the lactic dehydrogenase virus (LDV) and cell-virus relationships. Virology 26:512
    [Google Scholar]
  9. Gibbs A. J., Nixon H. L., Woods R. D. 1963; Properties of purified preparations of lucerne mosaic virus. Virology 19:441
    [Google Scholar]
  10. Gibbs A. J., Hecht-Poinar E., Woods R. D., Mckee R. K. 1966; Some properties of three related viruses: Andean potato latent, dulcamara mottle, and Ononis yellow mosaic. J. gen. Microbiol 44:177
    [Google Scholar]
  11. Harrison B. D., Nixon H. L. 1959; Separation and properties of particles of tobacco rattle virus with different lengths. J. gen. Microbiol 21:569
    [Google Scholar]
  12. Johnston J. P., Ogston A. G. 1946; A boundary anomaly found in the ultracentrifugal sedimentation of mixtures. Trans. Faraday Soc 42:789
    [Google Scholar]
  13. Kelley J. J., Kaesberg P. 1962; Biophysical and biochemical properties of top componenta and bottom component of alfalfa mosaic virus. Biochim. biophys. Acta 61:865
    [Google Scholar]
  14. Leberman R. 1965; Use of uranyl formate as a negative stain. J. molec. Biol 13:606
    [Google Scholar]
  15. Mansi W. 1958; Slide gel diffusion precipitin test. Nature; Lond: 1811289
    [Google Scholar]
  16. Markham R. 1955; Nucleic acids, their components and related compounds. In Modern Methods of Plant Analysis 4 p 246 Paech K., Tracey M. V. Ed New York: Springer Verlag N.Y Inc;
    [Google Scholar]
  17. Paul H. L. 1959; Die Bestimmung des Nucleinsäuregehaltes pflanzlicher Viren mit Hilfe einer spektrophotometrischen Methode. Z. naturf 14:427
    [Google Scholar]
  18. Polson A. 1958; A method for determination of diffusion coefficients by the gel-precipitin technique. Biochim. biophys. Acta 29:426
    [Google Scholar]
  19. Reichmann M. E. 1965; Determination of ribonucleic acid content of spherical viruses from sedimentation coefficients of full and empty particles. Virology 25:166
    [Google Scholar]
  20. Steere R. L. 1956; Purification and properties of tobacco ringspot virus. Phytopathology 46:60
    [Google Scholar]
  21. Wetter C. 1960; Partielle Reinigung einiger gestreckter Pflanzenviren und ihre Verwendung als Antigene bei der Immunisierung mittels Freundsehem Adjuvans. Arch. Mikrobiol 37:278
    [Google Scholar]
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