1887

Abstract

SUMMARY

Purified preparations of the golden elderberry strain of cherry leaf roll virus contain two spherical nucleoprotein components with sedimentation coefficients of 115s and 128s. Purification of the components by centrifuging several times through sucrose density gradients decreased their specific infectivity. The original specific infectivity of unfractionated virus was restored by mixing the separated components, indicating that both components are necessary for maximum infectivity. The components were indistinguishable serologically, in their extinction spectra and in their appearance in the electron microscope. Polyacrylamide gel electrophoresis of preparations from each component indicated that both contained a single protein species of 54,000 mol. wt, but that whereas the 115 component contained a RNA molecule of 2.1 × 10 mol. wt the 128 component contained one of 2.4 × 10. These and other properties suggest that cherry leaf roll virus can be considered a member of the nepovirus group. The present cryptogram of the golden elderberry strain is R/1 : 2.1/40 + 2.4/43 : S/S : S/*.

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1972-09-01
2024-05-13
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References

  1. BARNETT O. W., MURANT A. F. 1970; Host range, properties and purification of raspberry bushy dwarf virus. Annals of Applied Biology 65:435
    [Google Scholar]
  2. CADMAN C. H. 1963; Biology of soil-borne viruses. Annual Review of Phytopathology 1:143
    [Google Scholar]
  3. CROPLEY R. 1961; Cherry leaf roll virus. Annals of Applied Biology 49:524
    [Google Scholar]
  4. CROPLEY R., TOMLINSON J. A. 1971; Cherry leaf roll virus. Commonwealth Mycological Institute) Association of Applied Biologists Descriptions of Plant Viruses no. 80
    [Google Scholar]
  5. DIENER T. O., SCHNEIDER I. R. 1966; The two components of tobacco ringspot virus nucleic acid: origin and properties. Virology 29:100
    [Google Scholar]
  6. FLEGG J. J. M. 1969; Tests with potential nematode vectors of cherry leaf-roll virus. Report of East Mailing Research Station 1968:155
    [Google Scholar]
  7. FRITZCHE R., KEGLER H. 1964; Die Übertragung des Blattrollvirus der Kirsche (cherry leaf-roll virus) durch Nematoden. Naturwissenschaften 51:299
    [Google Scholar]
  8. GOODING G. V. 1963; Purification and serology of a virus associated with the grape yellow vein disease. Phytopathology 53:475
    [Google Scholar]
  9. HANSEN A. J. 1967; A virus from golden elderberry. Phytopathology 57:813 abstract
    [Google Scholar]
  10. HANSEN A. J., STACE-SMITH R. 1971a; Determination of a virus in golden elderberry. Proceedings for the 8th European Symposium on Fruit Tree Virus DiseasesBordeaux1970 Annales der Phytopathologie Numero hors serie 1971 147
    [Google Scholar]
  11. HANSEN A. J., STACE-SMITH R. 1971b; Properties of a virus isolated from golden elderberry. Sambucus nigra aurea. Phytopathology 61:1222
    [Google Scholar]
  12. HARRISON B. D., FINCH J. T., GIBBS A. J., HOLLINGS M., SHEPHERD R. J., VALENTA V., WETTER C. 1971; Sixteen groups of plant viruses. Virology 45:356
    [Google Scholar]
  13. HARRISON B. D., MURANT A. F., MAYO M. A. 1972; Evidence for two functional RNA species in raspberry ringspot virus. Journal of General Virology 16:339
    [Google Scholar]
  14. HARRISON B. D., WOODS R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610
    [Google Scholar]
  15. JONES A. T., MURANT A. F. 1971; Serological relationship between cherry leaf roll, elm mosaic and golden elderberry viruses. Annals of Applied Biology 69:11
    [Google Scholar]
  16. LOENING U. E. 1967; The fractionation of high molecular weight ribonucleic acid by Polyacrylamide gel electrophoresis. Biochemical Journal 102:251
    [Google Scholar]
  17. LOENING U. E. 1968; Molecular weight of ribosomal RNA in relation to evolution. Journal of Molecular Biology 38:355
    [Google Scholar]
  18. LOENING U. E., INGLE I. 1967; Diversity of RNA components in green plant tissues. Nature, London 215:363
    [Google Scholar]
  19. MARKHAM R. 1967a The ultracentrifuge. Methods in Virology Edited by Maramorosch K., Koprowski H. III New York and London: Academic Press;
    [Google Scholar]
  20. MARKHAM R. 1967b Diffusion. Methods in Virology Edited by Maramorosch K., Koprowski H. 11275 New York and London: Academic Press;
    [Google Scholar]
  21. MATTHEWS R. E. F. 1970 Plant Virology226 New York and London: Academic Press;
    [Google Scholar]
  22. MAYO M. A., MURANT A. F., HARRISON B. D. 1971; New evidence on the structure of nepoviruses. Journal of General Virology 12:175
    [Google Scholar]
  23. MURANT A. F., MAYO M. A., HARRISON B. D., GOOLD R. A. 1972; Properties of virus and RNA components of raspberry ringspot virus. Journal of General Virology 16:327
    [Google Scholar]
  24. SCHNEIDER I. R., DIENER T. O. 1966; The correlation between the proportions of virus-related products and the infectious component during synthesis of tobacco ringspot virus. Virology 39:92
    [Google Scholar]
  25. SCHNEIDER I. R., DIENER T. O. 1968; In vivo and in vitro decline of specific infectivity of tobacco ringspot virus correlated with nucleic acid degradation. Virology 35:150
    [Google Scholar]
  26. SEHGAL O. P., JEAN J. L., BHALLA R. B., SOONG M. M., KRAUSE G. F. 1970; Correlation between buoyant density and ribonucleic acid content in viruses. Phytopathology 60:1778
    [Google Scholar]
  27. STACE-SMITH R. 1966; Purification and properties of tomato ringspot virus and an RNA-deficient component. Virology 29:240
    [Google Scholar]
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