1887

Abstract

Production of interferon from cultured cells has required the presence of serum in the cell culture medium although it may be omitted during the period of interferon induction. In the system reported here a fully defined medium, free of protein, is employed. This permits the culture of the cells and all phases of interferon induction and release to be studied under completely defined conditions. The final product can easily be separated from its inducers to yield a preparation of high specific activity.

The strain of mouse fibroblasts, adapted to grow in suspension, has been under regular culture in this laboratory (Griffiths & Pirt, 1967; Birch & Pirt, 1969, 1970, 1971; Blaker & Pirt, 1971) in the protein-free medium (Birch & Pirt, 1970). The only polymer used in the medium is the uncharged polysaccharide methylcellulose.

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1972-07-01
2024-12-13
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References

  1. BIRCH J. R., PIRT S. J. 1969; The choline and serum protein requirements of mouse fibroblast cells (strain LS) in culture. Journal of Cell Science 5:135
    [Google Scholar]
  2. BIRCH J. R., PIRT S. J. 1970; Improvements in a chemically defined medium for the growth of mouse cells (strain LS) in suspension. Journal of Cell Science 7:661
    [Google Scholar]
  3. BIRCH J. R., PIRT S. J. 1971; The quantitative glucose and mineral requirements of mouse LS (suspension) cells in a chemically defined medium. Journal of Cell Science 8:693
    [Google Scholar]
  4. BLAKER G. J., PIRT S. J. 1971; The uptake of vitamins by mouse fibroblast cells (strain LS) during growth on a chemically defined medium. Journal of Cell Science 8:701
    [Google Scholar]
  5. BUCK K. W., CHAIN E. B., HIMMELWEIT F. 1971; A comparison of interferon induction in mice by purified Penicillium chrysogenum virus and derived double-stranded RNA. Journal of General Virology 12:131
    [Google Scholar]
  6. DIANZANI F., CANTAGALLI P., GAGNONI S., RITA G. 1968; Effect of DEAE-dextran on production of interferon induced by synthetic double-stranded RNA in L cell cultures. Proceedings of the Society for Experimental Biology and Medicine 128:708
    [Google Scholar]
  7. DIANZANI F., GAGNONI S., CANTAGALLI P. 1970; Studies on the potentiating effect of DEAE-dextran on interferon production induced by double-stranded synthetic polynucleotides. Annals of the New York Academy of Sciences 173:727
    [Google Scholar]
  8. FALCOFF E., FALCOFF R., FOURNIER F., CHANY C. 1966; Production en masse, purification partielle et caracterisation d’un interferon destine des essais therapeutique humains. Annales de l’Institut Pasteur III:562
    [Google Scholar]
  9. FIELD A. K., TYTTEL A. A., LAMPSON G. P., HILLEMAN M. R. 1967; Inducers of interferon and host resistance: II. Multistranded synthetic polynucleotide complexes. Proceedings of the National Academy of Sciences of the United States of America 58:1004
    [Google Scholar]
  10. FINNEY D. J. 1952 Statistical method in biological assay chapter 18 London: Charles Griffin & Company, Ltd;
    [Google Scholar]
  11. FINTER N. B. 1969; Dye uptake methods of assessing viral cytopathogenicity and their application to interferon assays. Journal of General Virology 5:419
    [Google Scholar]
  12. GRIFFITHS J. B., PIRT S. J. 1967; The uptake of amino acids by mouse cells (strain LS) during growth in batch culture and chemostat culture: the influence of cell growth rate. Proceedings of the Royal Society B 168:421
    [Google Scholar]
  13. LOCKART R. Z. 1966 Biological properties of interferons. Criteria for acceptance of a viral inhibitor as an interferon. The Interferons Ed. by Finter N. B. Amsterdam; North Holland Publishing Company:
    [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265
    [Google Scholar]
  15. MERIGAN T. C, WINGET C. A., DIXON C B. 1965; Purification and characterisation of vertebrate interferons. Journal of Molecular Biology 13:679
    [Google Scholar]
  16. TILLES J. G. 1970; Diethylaminoethyl-dextran enhancement of interferon induction by a complexed polyribonucleotide. Proceedings of the Society for Experimental Biology and Medicine 133:1334
    [Google Scholar]
  17. YOUNGNER J. S., SCOTT A. W., HALLUM J. V., STINEBRING W. R. 1966; Interferon induction by inactivated Newcastle disease virus in cell cultures and mice. Journal of Bacteriology 92:862
    [Google Scholar]
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