A direct study of the complement-fixing reactivity of cell membranes, soluble components of cell membranes and cell interiors of lymphoblast cell lines showed that with highly concentrated levels of antigen, complement-fixing reactivity could be measured consistently between the cell interior of lymphoma (IM), leukaemic (4265) and Burkitt lymphoma (P) cultured cells and sera from patients with infectious mononucleosis or Burkitt lymphoma. A measurable complement-fixing reactive cell-membrane antigen appeared to be present in IM and 4265 cells. Complement-fixing activity was also recovered in a soluble fraction of 4265 cell membranes after sonication and separation by Sephadex gel filtration. Separation of the complement-fixing reactive component from HL-A antigens was achieved by gel electrophoresis.


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