1887

Abstract

Summary

A satisfactory purification procedure for oat necrotic mottle virus involved clarification of the plant juice by silver nitrate, followed by two cycles of differential centrifugation, and then rate-zonal density gradient centrifugation. The concentrate was finally passed through a column of agarose gel. Antiserum to clarified sap from virus-free oats did not react with the final preparation. A medium consisting of 0.1 -sodium citrate buffer, pH 6.2, containing 0.5 -urea, was suitable for the concentrated virus. The virus particles were filamentous with a diameter of 11 nm. and a normal length of 720 nm.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-12-3-259
1971-09-01
2019-10-19
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-12-3-259
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error