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Abstract

Colistin is a last resort antimicrobial used for the treatment of gram-negative bacterial infections. Plasmid-mediated colistin resistance () genes are a cause of global concern, and, thus far, have been identified. In a previous study, we screened in derived from diseased pigs in Japan and reported a high prevalence of , and . However, the previous report on the prevalence of genes was inaccurate. In the present study, we aimed to clarify the prevalence of all reported variants of in derived from the diseased pigs, which were previously screened for . Additionally, we also characterized the -positive , which was detected in this study. We screened in 120 strains from diseased pigs and -positive and an -carrying plasmid were also characterized. One -positive colistin-susceptible strain was detected (0.8 %). Plasmid-mediated was transferred to ML4909 as the recipient strain, and it was located on IncHI2/HI2A plasmid p387_L with other antimicrobial resistance genes (ARGs). The region harbouring ARGs including was similar to that on the chromosome harbouring isolated in Japan. , and were detected (4.2 %) in colistin-susceptible strains. was found to be disseminated via the plasmid IncHI2/HI2A p387_L and transferred and inserted into chromosomes via a transposon. Our results suggest that genes should be monitored regularly, regardless of their susceptibility to colistin.

Keyword(s): colistin , Escherichia coli , mcr and plasmid
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2022-11-28
2024-04-13
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