1887

Abstract

This study investigated the antiviral activity of betacyanins from red pitahaya () and red spinach () against dengue virus type 2 (DENV-2). The pulp of red pitahaya and the leaves of red spinach were extracted using methanol followed by sub-fractionation and Amberlite XAD16N column chromatography to obtain betacyanin fractions. The half maximum cytotoxicity concentration for betacyanin fractions from red pitahaya and red spinach on Vero cells were 4.346 and 2.287 mg ml, respectively. The half-maximal inhibitory concentration (IC) of betacyanin fraction from red pitahaya was 125.8 μg ml with selectivity index (SI) of 5.8. For betacyanin fraction from red spinach, the IC value was 14.62 µg ml with SI of 28.51. Using the maximum non-toxic betacyanin concentration, direct virucidal effect against DENV-2 was obtained from betacyanin fraction from red pitahaya (IC of 126.70 μg ml; 95.0 % virus inhibition) and red spinach (IC value of 106.80 μg ml; 65.9 % of virus inhibition). Betacyanin fractions from red pitahaya and red spinach inhibited DENV-2 .

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2019-10-31
2019-11-14
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References

  1. Rodenhuis-Zybert IA, Wilschut J, Smit JM. Dengue virus life cycle: viral and host factors modulating infectivity. Cell Mol Life Sci 2010;67: 2773– 2786 [CrossRef]
    [Google Scholar]
  2. Abd Kadir SL, Yaakob H, Mohamed Zulkifli R. Potential anti-dengue medicinal plants: a review. J Nat Med 2013;67: 677– 689 [CrossRef]
    [Google Scholar]
  3. Abdul Ahmad SA, Palanisamy UD, Tejo BA, Chew MF, Tham HW et al. Geraniin extracted from the rind of Nephelium lappaceum binds to dengue virus type-2 envelope protein and inhibits early stage of virus replication. Virol J 2017;14: 229 [CrossRef]
    [Google Scholar]
  4. Guzman MG, Alvarez M, Halstead SB. Secondary infection as a risk factor for dengue hemorrhagic fever/dengue shock syndrome: an historical perspective and role of antibody-dependent enhancement of infection. Arch Virol 2013;158: 1445– 1459 [CrossRef]
    [Google Scholar]
  5. Hu K, Thoens C, Bianco S, Edlund S, Davis M et al. The effect of antibody-dependent enhancement, cross immunity, and vector population on the dynamics of dengue fever. J Theor Biol 2013;319: 62– 74 [CrossRef]
    [Google Scholar]
  6. Lim SK, Lee YS, Namkung S, Lim JK, Yoon IK. Prospects for dengue vaccines for travelers. Clin Exp Vaccine Res 2016;5: 89– 100 [CrossRef]
    [Google Scholar]
  7. Choo WS. Betalains: Application in functional foods In Mérillon J-M, Ramawat KG. (editors) Bioactive Molecules in Food Cham: Springer International Publishing; 2018; pp 1– 28
    [Google Scholar]
  8. Gengatharan A, Dykes GA, Choo WS. Betalains: natural plant pigments with potential application in functional foods. LWT - Food Science and Technology 2015;64: 645– 649 [CrossRef]
    [Google Scholar]
  9. Soskova V. Reproduction of sugar beet mosaic and tobacco mosaic viruses in anthocyanized beet plants. Biol Plant 1970;12: 424– 427 [CrossRef]
    [Google Scholar]
  10. Feinberg MB, Ahmed R. Advancing dengue vaccine development. Science 2017;358: 865– 866 [CrossRef]
    [Google Scholar]
  11. Yong YY, Dykes G, Lee SM, Choo WS. Biofilm inhibiting activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms. J Appl Microbiol 2019;126: 68– 78 [CrossRef]
    [Google Scholar]
  12. Cai Y, Sun M, Wu H, Huang R, Corke H. Characterization and quantification of betacyanin pigments from diverse Amaranthus species. J Agric Food Chem 1998;46: 2063– 2070 [CrossRef]
    [Google Scholar]
  13. Yong YY, Dykes G, Lee SM, Choo WS. Comparative study of betacyanin profile and antimicrobial activity of red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius). Plant Foods Hum Nutr 2017;72: 41– 47 [CrossRef]
    [Google Scholar]
  14. Gengatharan A, Dykes GA, Choo W-S. The effect of pH treatment and refrigerated storage on natural colourant preparations (betacyanins) from red pitahaya and their potential application in yoghurt. LWT – Food Sci Technol 2017;80: 437– 445 [CrossRef]
    [Google Scholar]
  15. Koishi AC, Zanello PR, Bianco Éverson Miguel, Bordignon J, Nunes Duarte dos Santos C. Screening of dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay. PLoS One 2012;7: e51089 [CrossRef]
    [Google Scholar]
  16. Timiryasova TM, Bonaparte MI, Luo P, Zedar R, Hu BT et al. Optimization and validation of a plaque reduction neutralization test for the detection of neutralizing antibodies to four serotypes of dengue virus used in support of dengue vaccine development. Am J Trop Med Hyg 2013;88: 962– 970 [CrossRef]
    [Google Scholar]
  17. Calabrese EJ, Baldwin LA. Hormesis: The dose-response revolution. Annu Rev Pharmacol Toxicol 2003;43: 175– 197 [CrossRef]
    [Google Scholar]
  18. Yong YY, Dykes G, Lee SM, Choo WS. Effect of refrigerated storage on betacyanin composition, antibacterial activity of red pitahaya (Hylocereus polyrhizus) and cytotoxicity evaluation of betacyanin rich extract on normal human cell lines. LWT 2018a;91: 491– 497 [CrossRef]
    [Google Scholar]
  19. Khan MI, Safety Plant betalains:. Antioxidant activity, clinical efficacy, and bioavailability. Compr Rev Food Sci F 2016;15: 316– 330
    [Google Scholar]
  20. Hassandarvish P, Rothan HA, Rezaei S, Yusof R, Abubakar S et al. In silico study on baicalein and baicalin as inhibitors of dengue virus replication. RSC Adv 2016;6: 31235– 31247 [CrossRef]
    [Google Scholar]
  21. Senthilvel P, Lavanya P, Kumar KM, Swetha R, Anitha P et al. Flavonoid from Carica papaya inhibits NS2B-NS3 protease and prevents Dengue 2 viral assembly. Bioinformation 2013;9: 889– 895 [CrossRef]
    [Google Scholar]
  22. Chen Y, Maguire T, Marks RM. Demonstration of binding of dengue virus envelope protein to target cells. J Virol 1996;70: 8765– 8772
    [Google Scholar]
  23. Chen Y, Maguire T, Hileman RE, Fromm JR, Esko JD et al. Dengue virus infectivity depends on envelope protein binding to target cell heparan sulfate. Nat Med 1997;3: 866– 871 [CrossRef]
    [Google Scholar]
  24. Zandi K, Lim TH, Rahim NA, Shu MH, Teoh BT et al. Extract of Scutellaria baicalensis inhibits dengue virus replication. BMC Complement Altern Med 2013;13: 91 [CrossRef]
    [Google Scholar]
  25. Zandi K, Teoh BT, Sam SS, Wong PF, Mustafa MR et al. Antiviral activity of four types of bioflavonoid against dengue virus type-2. Virol J 2011;8: 560 [CrossRef]
    [Google Scholar]
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