1887

Access Microbiology open research platform logo

Volume 3, Issue 10

Antimicrobial properties of phytohormone (gibberellins) against phytopathogens and clinical pathogens Open Access

Abstract

The antimicrobial potential of physiologically active diterpenoid plant-derived gibberellins (gibberellic acids; GAs) was tested on microbial pathogens of significance to plant and human health. The racemic enantiomer GA3 produced varying inhibitory effects against a wide range of plant host disease causal agents (phytopathogens) comprising fungi, oomycetes and bacteria. The results showed that GA3 effected either strong growth arrest of phytopathogenic fungi or holistic biocidal effects on oomycete and phytopathogenic fungi at higher concentration (>10–50 mM) and increased hyphal extension growth when the concentration of GA3 was lowered (<10−0.1 mM). When human clinical pathogenic bacteria cohorts were challenged with gibberellin enantiomers, viz GA1, GA4, GA5, GA7, GA9 and GA13 (50 mM), employing Kirby–Bauer disc bioassay methods for assessment of their efficacies, no inhibitory effect was seen with gibberellin enantiomers, viz GA1, GA3, GA5 and GA13, while GA4 inhibited all human clinical bacterial organisms examined, with GA and GA showing limited activity. The antibiotic effects of enantiomeric diterpenoid phytohormones evinced in our preliminary study raise prospects for further studies to fully examine their potential therapeutic value for human healthcare and their compliance with cytotoxicity and other ethical considerations in the future.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): AMR , antimicrobial resistance , cystic fibrosis , gibberellin , plant pathogens and Pseudomonas
Funding
This study was supported by the:
  • deara (Award 16/3/11)
    • Principle Award Recipient: JRRao
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
Loading

Article metrics loading...

/content/journal/acmi/10.1099/acmi.0.000278
2021-10-20
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/acmi/3/10/acmi000278.html?itemId=/content/journal/acmi/10.1099/acmi.0.000278&mimeType=html&fmt=ahah

References

  1. MacMillan J, Takahashi N. Proposed procedure for the allocation of trivial names to the Gibberellins. Nature 1968; 217:170–171 [View Article] [PubMed]
     [Google Scholar]
  2. Calvo P, Nelson L, Kloepper JW. Agricultural uses of plant biostimulants. Plant Soil 2014; 383:3–41 [View Article]
     [Google Scholar]
  3. Saa S, Olivos-Del Rio A, Castro S, Brown PH. Foliar application of microbial and plant based biostimulants increases growth and potassium uptake in almond (Prunus dulcis [Mill.] D. A. Webb. Front Plant Sci 2015; 6:87 [View Article] [PubMed]
     [Google Scholar]
  4. Sharma HSS, Fleming C, Selby C, Rao JR, Martin T. Plant biostimulants: a review on the processing of macroalgae and use of extracts for crop management to reduce abiotic and biotic stresses. J Appl Phycol 2013; 26:465–490 [View Article]
     [Google Scholar]
  5. Nelson D, Beattie K, McCollum G, Martin T, Sharma S et al. Performance of Natural Antagonists and Commercial Microbiocides towards in Vitro Suppression of Flower Bed Soil-Borne Fusarium oxysporum . Adv Microbiol 2014; 04:151–159 [View Article]
     [Google Scholar]
  6. Xie L, Chen Y, Chen J, Zhang H, Liao Y et al. Anti-tumor effects and mechanism of GA-13315, a novel gibberellin derivative, in human lung adenocarcinoma: An in vitro and in vivo study. Cell Mol Biol Lett 2019; 24:6 [View Article] [PubMed]
     [Google Scholar]
  7. Hedden P, Sponsel V. A century of gibberellin research. J Plant Growth Regul 2015; 34:740–760 [View Article] [PubMed]
     [Google Scholar]
  8. Efstratiou E, Hussain AI, Nigam PS, Moore JE, Ayub MA et al. Antimicrobial Activity of Callendula officinalis petal extracts against fungi, as well as Gram-ve and Gram+ve clinical pathogens Complementary Therapies in Clinical Practice 2012 pp 173–176
     [Google Scholar]
  9. Eriksson S, Böhlenius H, Moritz T, Nilsson O. Ga4 is the active gibberellin in the regulation of leafy transcription and arabidopsis floral initiation. Plant Cell 2006; 18:2172–2181 [View Article] [PubMed]
     [Google Scholar]
  10. Rodriguez R, Redman R. More than 400 million years of evolution and some plants still can’t make it on their own: plant stress tolerance via fungal symbiosis. J Exp Bot 2008; 59:1109–1114 [View Article] [PubMed]
     [Google Scholar]
  11. Arteca RN. Plant Growth Substances - Principles and Applications New York: Chapman & Hall; 1995 p 332
     [Google Scholar]
  12. Sponsel VM, Hedden P. Gibberellin biosynthesis and metabolism. Davies PJ. eds In Plant hormones Dordrecht: Kluwer Academic Publishers; 2004 pp 62–98
     [Google Scholar]
  13. Hearst R, Nelson D, McCollum G, Millar BC, Maeda Y et al. An examination of antibacterial and antifungal properties of constituents of shiitake (lentinula edodes) and oyster (pleurotus ostreatus) mushrooms. Complement Ther Clin Pract 2009; 15:5–7 [View Article]
     [Google Scholar]
  14. Moore JE, McCollum G, Murphy A, Millar BC, Nelson D et al. Assessment of inhibition/growth-promoting properties of new agents on moulds: description of a simple bio-imaging technique. Br J Biomed Sci 2010; 67:145–146 [View Article] [PubMed]
     [Google Scholar]
  15. Woods-Panzaru S, Nelson D, McCollum G, Ballard LM, Millar BC et al. An examination of antibacterial and antifungal properties of constituents described in traditional Ulster cures and remedies. Ulster Med J 2009; 78:13–15 [PubMed]
     [Google Scholar]
  16. Hoffman JIE. Linear Regression: Comparison Problem (Advanced), setting standard error confidence intervals using Altman-Bland (1999, 2009) estimates. In Biostatistics for Practitioners USA: Academic Press; 2015 pp 451–500
     [Google Scholar]
  17. Carmichael E, Rao JR. Evaluation of thermogravimetric analysis as a rapid tool for the detection of rhizobacteria biostimulants used in precision agriculture. Journal of Applied Biology & Biotechnology 2021; 9:8–16
     [Google Scholar]
  18. Selby C, Carmichael E, Shekhar Sharma HS. Bio-refining of perennial ryegrass (Lolium perenne): evaluation of aqueous extracts for plant defence elicitor activity using French bean cell suspension cultures. Chemical and Biological Technologies in Agriculture 2016; 3:2–7 [View Article]
     [Google Scholar]
  19. Sharma HSS, Selby C, Carmichael E, McRoberts C, Rao JR et al. Physicochemical analyses of plant biostimulant formulations and characterisation of commercial products by instrumental techniques. Chem Biol Biol Tech Agriculture 2016; 3:1–17
     [Google Scholar]
  20. EMC (Electronic Medicines Compendium) Amoxicillin. Summary of Product Characteristics; 2019 https://www.medicines.org.uk accessed 22 Jun 2021
  21. Nicolas I, Bordeau V, Bondon A, Baudy-Floc’h M, Felden B. novel antibiotics effective against gram-positive and -negative multi-resistant bacteria with limited resistance. PLoS Biol 2019; 17:e3000337 [View Article] [PubMed]
     [Google Scholar]
  22. Hamayun M, Khan SA, Iqbal I, Ahmad B, Lee I-. J. Isolation of a Gibberellin-producing fungus (Penicillium sp. MH7) and Growth Promotion of Crown Daisy (Chrysanthemum coronarium). Microbiology and Biotechnology 2010; 20:207
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/acmi/10.1099/acmi.0.000278
Loading
Antimicrobial properties of phytohormone (gibberellins) against phytopathogens and clinical pathogens
Access Microbiology 3, 000278 (2021); https://doi.org/10.1099/acmi.0.000278
/content/journal/acmi/10.1099/acmi.0.000278
/content/journal/acmi/10.1099/acmi.0.000278
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error