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Abstract

Melioidosis is an infection that most commonly presents with bacteraemia. Culture-based laboratory methods can result in a significant delay to organism identification. Molecular diagnostic techniques have a high sensitivity and rapid time to diagnosis. A decreased time to diagnosis is likely to improve patient outcomes.

To compare the Panther Fusion automated molecular instrument to an in-house method for the detection of directly from spiked human whole-blood samples.

The in-house method detected 11/12 (92 %) samples with a concentration of 2.5–4.5×10 c.f.u. ml. The Panther was less reliable, detecting only 8/14 (75 %) samples with a similar bacterial concentration. The Panther was able to detect 12/12 (100 %) spiked blood culture-positive samples.

The direct detection of from patient blood on presentation to a healthcare facility will significantly decrease time to diagnosis. We describe an in-house real-time PCR method with the lowest reported limit of detection to date. Due to lower sensitivity, the Panther Fusion would be best used as a diagnostic method directly from a positive blood culture.

Funding
This study was supported by the:
  • Pathology Queensland (Award SERC 6096)
    • Principle Award Recipient: IanGassiep
  • Hologic
    • Principle Award Recipient: IanGassiep
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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/content/journal/acmi/10.1099/acmi.0.000333
2022-03-21
2024-11-11
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