Chinese herbal medicines and nutraceuticals inhibit Pseudomonas aeruginosa biofilm formation

Minami Hayashi; Hiroshi Kaneko; Tetsuya Yamada; Hideaki Ikoshi; Norihisa Noguchi; Hidemasa Nakaminami

doi:10.1099/acmi.0.000254

Volume 3, Issue 8

Short Communication

Open Access

Chinese herbal medicines and nutraceuticals inhibit Pseudomonas aeruginosa biofilm formation

Minami Hayashi¹, Hiroshi Kaneko¹, Tetsuya Yamada², Hideaki Ikoshi², Norihisa Noguchi¹ and Hidemasa Nakaminami¹
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Affiliations: ¹ Department of Clinical Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, Japan ² Department of Traditional Chinese Medicine, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, Japan
*Correspondence: Hidemasa Nakaminami, [email protected]
Published: 17 August 2021 https://doi.org/10.1099/acmi.0.000254

Abstract

Pseudomonas aeruginosa is a major biofilm-forming, opportunistic pathogen. Tolerance to antimicrobial agents due to biofilm formation may lead to the emergence of antimicrobial-resistant bacterial strains. Thus, adjunctive agents that can inhibit biofilm formation are necessary to enhance the therapeutic efficacy of antimicrobial agents. In this study, we evaluated the anti-biofilm formation activity of selected Chinese herbal medicines and nutraceuticals, which are commercially available in Japan. Among the eight agents evaluated for their potential to inhibit biofilm formation, Eiekikaryu S, Iribakuga and Hyakujunro significantly reduced P. aeruginosa biofilm formation (P <0.05) without inhibiting bacterial growth. Additionally, the expression of biofilm-associated genes (rhlR, rhlA and lasB) in P. aeruginosa was significantly suppressed by Eiekikaryu S, Iribakuga and Hyakujunro (P <0.001). Our findings indicate that some Chinese herbal medicines and nutraceuticals can be potential adjunctive agents for antimicrobial therapy against P. aeruginosa .

Received: 24/01/2021
Accepted: 30/06/2021
Published Online: 17/08/2021

Keyword(s): biofilm , Chinese herbal medicine , nutraceutical and Pseudomonas aeruginosa

Funding

This study was supported by the:

Japan Society for the Promotion of Science (Award JP18H00370)
- Principle Award Recipient: NorihisaNoguchi

This is an open-access article distributed under the terms of the Creative Commons Attribution License.

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Chinese herbal medicines and nutraceuticals inhibit Pseudomonas aeruginosa biofilm formation

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Volume 3, Issue 8

Short Communication

Open Access

Chinese herbal medicines and nutraceuticals inhibit Pseudomonas aeruginosa biofilm formation

Abstract

Funding

Supplementary material 1

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