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Volume 3, Issue 3

Study of quorum-sensing and genes and their dependent virulence factors in isolates from infected burn wounds Open Access

Abstract

is an opportunistic pathogen responsible for burn-wound infection. High incidence, infection severity and increasing resistance characterize -induced burn infection.

To estimate quorum-sensing (QS)-dependent virulence factors of isolates from burn wounds and correlate it to the presence of QS genes.

A cross-sectional descriptive study included 50 . isolates from burn patients in Mansoura University Plastic and Burn Hospital, Egypt. Antibiotic sensitivity tests were done. All isolates were tested for their ability to produce biofilm using a micro-titration assay method. Protease, pyocyanin and rhamnolipid virulence factors were determined using skimmed milk agar, King’s A medium and CTAB agar test, respectively. The identity of QS and genes was confirmed using PCR.

In total, 86 % of isolates had proteolytic activity. Production of pyocyanin pigment was manifested in 66 % of isolates. Altogether, 76 % of isolates were rhamnolipid producers. Biofilm formation was detected in 96 % of isolates. QS and genes were harboured by nearly all isolates except three isolates were negative for both and genes and two isolates were positive for gene and negative for gene. Forty-nine isolates were considered as extremely QS-proficient strains as they produced QS-dependent virulence factors. In contrast, one isolate was a QS deficient strain.

QS affects virulence-factor production and biofilm in burn wounds. Isolates containing and seem to be a crucial regulator of virulence factors and biofilm formation in whereas the gene positively regulates biofilm formation, proteolytic activity, pyocyanin production and rhamnolipid biosurfactant synthesis. The QS regulatory gene affects protease and rhamnolipid production positively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biofilm , Pseudomonas aeruginosa , QS and virulence
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2021-03-05
2024-04-26
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Study of quorum-sensing LasR and RhlR genes and their dependent virulence factors in Pseudomonas aeruginosa isolates from infected burn wounds
Access Microbiology 3, 000211 (2021); https://doi.org/10.1099/acmi.0.000211
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