1887

Abstract

A strain deficient in both its major proteases, intracellular (ClpP) and extracellular (HtrA), was constructed and characterized. This strain, hereafter called , could be obtained only by conjugation between a donor strain and an recipient strain in the NZ9000 context, allowing heterologous gene expression under the control of the NICE (nisin-controlled expression) system. The double mutant showed both higher stress tolerance (e.g. high temperature and ethanol resistance) and higher viability than single or mutant strains. In addition, the secretion rate of two heterologous proteins (staphylococcal nuclease Nuc and Nuc-E7) was also higher in than in the wild-type strain. This strain should be a useful host for high-level production and quality of stable heterologous proteins.

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2006-09-01
2024-10-10
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