1887

Abstract

5-Methyl cytosine (m5C) was detected in genomic DNA of the enteric pathogen by HPLC analysis and immunoblotting with m5C-specific antibody. Although cleavage with the restriction endonuclease RII revealed the absence of a Dcm homologue in , analysis of the genome sequence indicated the presence of a gene, designated in this study as , which encodes a DNA (cytosine-5-)-methyltransferase (m5C-MTase) designated M.Vch. M.Vch is not associated with a restriction endonuclease or a mismatch very short patch repair (Vsr)-like endonuclease and is hence an ‘orphan’ or solitary MTase, although analysis of a phylogenetic tree indicated that related cytosine MTases are all components of restriction-modification systems. M.Vch recognizes and methylates the first 5′ C in the degenerate sequence 5′-RCCGGY-3′. RT-PCR analysis suggested that gene expression is increased during the stationary phase of growth. During stationary phase, the spontaneous mutation frequency in the wild-type strain was significantly higher than in the corresponding mutant strain, suggesting that the presence of M.Vch and the absence of a very short patch (VSP) repair-like system imposes upon a mutator phenotype.

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2006-04-01
2021-05-18
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