1887

Abstract

Silencing of , the second structural gene of the tricistronic levansucrase operon encoding the endolevanase LevB, decreases the level of levansucrase expression in . Conversely, independent expression of greatly stimulates operon expression. This autogenous effect is mediated by the transcript, which carries an internal sequence (5′-AAAGCAGGCAA-3′) involved in the enhancing effect. , the transcript displays an affinity for the N-terminal fragment of SacY ( 0·2 μM), the regulatory protein that prevents transcription termination of the levansucrase operon. This positive-feedback loop leads to an increase in the operon expression when is growing in the presence of high sucrose concentrations. Under these conditions, extracellular levan synthesized by the fructosyl polymerase activity of levansucrase can be degraded mainly into levanbiose by the action of LevB. Levanbiose is neither taken up nor metabolized by the bacteria. This work modifies the present view of the status of levansucrase in physiology.

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2004-11-01
2019-11-14
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