1887

Abstract

The cAMP-dependent pathway, which regulates yeast-to-hypha morphogenesis in , is controlled by changes in cAMP levels determined by the processes of synthesis and hydrolysis. Both low- and high-affinity cAMP phosphodiesterases are encoded in the genome. , encoding the high-affinity cAMP phosphodiesterase, has been cloned and shown to be toxic in upon overexpression under p, but functional under the moderate p. Deletion of causes elevated cAMP levels and responsiveness to exogenous cAMP, higher sensitivity to heat shock, severe growth defects at 42 °C and highly reduced levels of transcription. in hypha-inducing liquid medium , deletion prohibits normal hyphal, but not pseudohyphal growth. On solid medium mutants form aberrant hyphae, with fewer branches and almost no lateral buds, which are deficient in hypha-to-yeast reversion. The phenotypic defects of mutants show that the cAMP-dependent pathway plays specific roles in hyphal and pseudohyphal development, its regulatory role however, being greater in liquid than on solid medium . The increased expression of after serum addition correlates well with a drop in cAMP levels following the initial rise in response to the hyphal inducer. These results suggest that Capde2p mediates a desensitization mechanism by lowering basal cAMP levels in response to environmental stimuli in . .

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2003-10-01
2019-10-15
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