1887

Abstract

Diverse types of genomic DNA organization have been found in , especially among species. Previous studies of concentrated mainly on biovar 1 strains. Little attention has been given to biovar 2 strains. The biovar 2 genome consists of a large, circular chromosome and second megabase-sized replicon, as well as several plasmids. In this study two biovar 2 strains were analysed, () K84 and A4, by constructing physical maps of their chromosomes and mega-replicons. The maps revealed that in both strains their chromosomes consist of approximately 3·7 Mbp, while the mega-replicons are 2·6 Mbp circular DNAs. Gene mapping and comparative genomic analysis were performed based on the physical maps using Southern hybridization. It was found that rDNA, as well as analysed virulence and virulence-related genes, are present only on the chromosomes. The inter-chromosomal relationship between biovar 1 and biovar 2 strains was also analysed. Interestingly, there was a high similarity between the chromosomes of biovar 2 and the circular chromosomes of biovar 1, whereas similarity among the smaller megabase-sized replicons was restricted to each biovar. Based on these observations the possible relationship among large replicons in biovars 1 and 2 is discussed.

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2003-10-01
2020-04-01
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References

  1. Casjens S.. 1998; The diverse and dynamic structure of bacterial genomes. Annu Rev Genet32:339–377
    [Google Scholar]
  2. De Costa D. M., Suzuki K., Satou M., Yoshida K.. 2001; Genome analysis of Agrobacterium tumefaciens : linkage map and genetic features of the left region of the linear chromosome. Genes Genet Syst76:363–371
    [Google Scholar]
  3. Galibert F., Finan T. M., Long S. R.. 53 other authors 2001; The composite genome of the legume symbiont Sinorhizobium meliloti . Science293:668–672
    [Google Scholar]
  4. Goodner B., Hinkle G., Gattung S.. 28 other authors 2001; Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58. Science294:2323–2328
    [Google Scholar]
  5. Itaya M., Tanaka T.. 1997; Experimental surgery to create subgenomes of Bacillus subtilis 168. Proc Natl Acad Sci U S A94:5378–5382
    [Google Scholar]
  6. Jumas-Bilak E., Michaux-Charachon S., Bourg G., Ramuz M., Allardet-Servent A.. 1998; Unconventional genomic organization in the alpha subgroup of the Proteobacteria . J Bacteriol180:2749–2755
    [Google Scholar]
  7. Kersters K., De Ley J.. 1984; Genus III. Agrobacterium Conn 1942. In Bergey's Manual of Systematic Bacteriology pp 244–254 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  8. McClure N. C., Ahmadi A., Clare B. G.. 1998; Construction of a range of derivatives of the biological control strain Agrobacterium tumefaciens K84: a study of factors involved in biological control of crown gall disease. Appl Environ Microbiol64:3977–3982
    [Google Scholar]
  9. Nishiguchi R., Takanami M., Oka A.. 1987; Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA4b. Mol Gen Genet206:1–8
    [Google Scholar]
  10. Sambrook J., Russell W. D.. 2001; Molecular Cloning: a Laboratory Manual , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Sawada H., Ieki H., Oyaizu H., Matsumoto S.. 1993; Proposal for rejection of Agrobacterium tumefaciens and revised description for the genus Agrobacterium and for Agrobacterium radiobacter and Agrobacterium rhizogenes . Int J Syst Bacteriol43:694–702
    [Google Scholar]
  12. Suzuki K., Hattori Y., Uraji M., Ohta N., Iwata K., Murata K., Kato A., Yoshida K.. 2000; Complete nucleotide sequence of a plant tumor-inducing Ti plasmid. Gene242:331–336
    [Google Scholar]
  13. Suzuki K., Iwata K., Yoshida K.. 2001; Genome analysis of Agrobacterium tumefaciens : construction of physical maps for linear and circular chromosomal DNAs, determination of copy number ratio and mapping of chromosomal virulence genes. DNA Res8:141–152
    [Google Scholar]
  14. Volff J. N., Altenbuchner J.. 2000; A new beginning with new ends: linearisation of circular chromosomes during bacterial evolution. FEMS Microbiol Lett186:143–150
    [Google Scholar]
  15. Wood D. W., Setubal J. C., Kaul R.. 48 other authors 2001; The genome of the natural genetic engineer Agrobacterium tumefaciens C58. Science294:2317–2323
    [Google Scholar]
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