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d-Glucose uptake in germinating wild-type Aspergillus nidulans conidia is an energy-requiring process mediated by at least two transport systems of differing affinities for glucose: a low-affinity system (K m∼1·4 mM) and a high-affinity system (K m∼16 μM). The low-affinity system is inducible by glucose; the high-affinity system is subject to glucose repression effected by the carbon catabolite repressor CreA and is absent in sorA3 mutant conidia, which exhibit resistance to l-sorbose toxicity. An intermediate-affinity system (K m∼400 μM) is present in sorA3 conidia germinating in derepressing conditions. creA derepressed mutants show enhanced sensitivity to l-sorbose. The high-affinity uptake system appears to be responsible for the uptake of this toxic sugar.
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