1887

Abstract

In order to further understand the different physiological states of the tubercle bacillus, a frame of reference was sought by first correlating the macromolecular compositions of with specific growth rates and also with the rates of protein synthesis. Data for DNA : protein : RNA were converted to the average amounts of DNA [ ], protein [ ] and RNA [ ] per cell. The specific growth rate was found to be directly proportional to / . The specific protein synthesis rate per average cell [ ] was shown to be directly proportional to the third power of the ratio / which reflects the ribosome concentration. The equations derived were shown apply to both (=1·73 h) and BCG (=0·029 h).

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2003-03-01
2019-10-21
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References

  1. Al-Karadagh, S., Kristensen, O. & Liljas, A. ( 2000; ). A decade of progress in understanding the structural basis of protein synthesis. Prog Biophys Mol Biol 73, 167–193.[CrossRef]
    [Google Scholar]
  2. Armstrong, J. A. & D'Arcy-Hart, P. ( 1971; ). Response of cultured macrophages to Mycobacterium tuberculosis, with observations on fusion of lysosomes with phagosomes. J Exp Med 134, 713–740.[CrossRef]
    [Google Scholar]
  3. Arnstein, H. R. V. & Cox, R. A. ( 1992; ). Protein Biosynthesis: in Focus. Oxford: Oxford University Press.
  4. Baldwin, W. W., Hirkish, M. A. & Koch, A. L. ( 1994; ). A change in a single gene of Salmonella typhimurium can dramatically change its buoyant density. J Bacteriol 176, 5001–5004.
    [Google Scholar]
  5. Bremer, H. & Dennis, P. P. ( 1996; ). Modulation of chemical composition and other parameters of the cell growth rate. In Escherichia coli and Salmonella: Cellular and Molecular Biology, 2nd edn, pp. 1553–1568. Editor by F. C. Neidhardt and others. Washington, DC: American Society for Microbiology.
  6. Brosch, R., Gordon, S. V., Eiglmeier, K., Garnier, T., Tekaia, F., Yeramian, E. & Cole, S. T. ( 2000; ). Genomics, biology, and evolution of the Mycobacterium tuberculosis complex. In Molecular Genetics of Mycobacteria, pp. 19–36. Edited by G. F. Hatfull & W. R. Jacobs, Jr. Washington, DC: American Society for Microbiology.
  7. Butcher, P. O., Sole, K. M. & Mangan, J. A. ( 1999; ). RNA extraction. In Molecular Mycobacteriology: Techniques and Clinical Applications, pp. 385–350. Edited by R. A. Ollar & N. O. Connell. New York: Marcel Dekker.
  8. Cole, S. T., Brosch, R., Parkhill, J. & 39 other authors. ( 1998; ). Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393, 537–544.[CrossRef]
    [Google Scholar]
  9. Colston, M. J. & Cox, R. A. ( 1999; ). Mycobacterial growth and dormancy. In Mycobacteria: Molecular Biology and Virulence, pp. 198–219. Edited by C. Ratledge & J. Dale. Oxford, UK: Blackwell Science.
  10. Cooper, S. ( 1988; ). Rate and topography of cell wall synthesis during the division cycle of Salmonella typhimurium. J Bacteriol 170, 422–430.
    [Google Scholar]
  11. Cooper, S. & Helmstetter, C. E. ( 1968; ). Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol 31, 519–540.[CrossRef]
    [Google Scholar]
  12. Diaper, J. P. & Edwards, C. ( 1994; ). Survival of Staphylococcus aureus in lakewater monitored by flow cytometry. Microbiology 140, 35–42.[CrossRef]
    [Google Scholar]
  13. Ferrari, G., Langen, H., Naito, M. & Pieters, J. ( 1999; ). A coat protein on phagosomes involved in the intracellular survival of mycobacteria. Cell 97, 435–447.[CrossRef]
    [Google Scholar]
  14. Flärdh, K., Cohen, P. S. & Kellenberg, S. ( 1992; ). Ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine Vibrio sp. strain CCUG 15956. J Bacteriol 174, 6780–6788.
    [Google Scholar]
  15. Gonzalez-y-Merchand, J. A., Colston, M. J. & Cox, R. A. ( 1999; ). Effects of growth conditions on expression of mycobacterial murA and tyrS genes and contributions of their transcripts to precursor rRNA synthesis. J Bacteriol 181, 4617–4627.
    [Google Scholar]
  16. Helmstetter, C. E. & Cooper, S. ( 1968; ). DNA synthesis during the division cycle of rapidly growing E. coli B/r. J Mol Biol 31, 507–518.[CrossRef]
    [Google Scholar]
  17. Hiriyanna, K. T. & Ramakrishnan, T. ( 1986; ). Deoxyribonucleic acid replication time in Mycobacterium tuberculosis H37 Rv. Arch Microbiol 144, 105–109.[CrossRef]
    [Google Scholar]
  18. Ingraham, J. L., Maaløe, O. & Neidhardt, F. C. ( 1983; ). Growth of the Bacterial Cell. Sunderland, MA: Sinauer Associates.
  19. Jacobs, W. R., Jr ( 2000; ). Mycobacterium tuberculosis: a once genetically intractable organism. In Molecular Genetics of Mycobacteria, pp. 1–16. Edited by G. F. Hatfull & W. R. Jacobs, Jr. Washington, DC: American Society for Microbiology.
  20. Jacobsen, H. ( 1974; ). PhD thesis. University of Copenhagen.
  21. Koch, A. L. ( 1979; ). Microbial growth in low concentrations of nutrients. In Strategies of Microbial Life in Extreme Environments, pp. 261–269. Edited by M. Shilo. Weinheim: Verlag Chemie.
  22. Maaløe, O. & Kjeldgaard, N. O. ( 1966; ). Control of Macromolecular Synthesis: a Study of DNA, RNA and Protein Synthesis in Bacteria. New York: W. A. Benjamin.
  23. Pape, T., Wintermeyer, W. & Rodnina, M. V. ( 1998; ). Complete kinetic mechanism of elongation factor Tu-dependent binding of aminoacyl-tRNA to the A-site of the E. coli ribosome. EMBO J 17, 7490–7497.[CrossRef]
    [Google Scholar]
  24. Pedersen, S., Bloch, P. L., Reeh, S. & Neidhardt, F. C. ( 1978; ). Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates. Cell 14, 179–190.[CrossRef]
    [Google Scholar]
  25. Powell, E. O. ( 1956; ). Growth rate and generation time of bacteria, with special reference to continuous culture. J Gen Microbiol 15, 492–511.[CrossRef]
    [Google Scholar]
  26. Ratledge, C. ( 1982; ). Nutrition, growth and metabolism. In Biology of the Mycobacteria, pp. 185–271. Edited by C. Ratledge & J. L. Stanford. London: Academic Press.
  27. Schaechter, M., Williamson, J. P., Hood, J. R., Jr & Koch, A. L. ( 1962; ). Growth, cell and nuclear divisions in some bacteria. J Gen Microbiol 29, 421–434.[CrossRef]
    [Google Scholar]
  28. Shepard, C. C. ( 1960; ). The experimental disease that follows the injection of human leprosy bacilli into the footpads of mice. J Exp Med 112, 445–454.[CrossRef]
    [Google Scholar]
  29. Turner, K., Porter, J., Pickup, R. & Edwards, C. ( 2000; ). Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry. J Appl Microbiol 89, 90–99.[CrossRef]
    [Google Scholar]
  30. Wayne, L. G. ( 1994; ). Cultivation of Mycobacterium tuberculosis for research purposes. In Tuberculosis: Pathogenesis, Protection and Control, pp. 73–83. Edited by B. Bloom. Washington, DC: American Society for Microbiology.
  31. Wayne, L. G. & Hayes, L. G. ( 1996; ). An in vitro model for sequential study of shift down of Mycobacterium tuberculosis through two stages of replicating persistence. Infect Immun 64, 2062–2069.
    [Google Scholar]
  32. Wayne, L. G. & Kubica, G. P. ( 1986; ). The mycobacteria. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1435–1457. Edited by P. H. A., Sneath, N. S., Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  33. Weber, I., Fritz, C., Ruttkowski, S., Kreft, A. & Bange, F. C. ( 2000; ). Anaerobic nitrate reductase (narGHJI) activity of Mycobacterium bovis BCG in vitro and its contribution to virulence in immunodeficient mice. Mol Microbiol 35, 1017–1025.[CrossRef]
    [Google Scholar]
  34. Wheeler, P. R. & Ratledge, C. ( 1994; ). Metabolism of Mycobacterium tuberculosis. In Tuberculosis; Pathogenesis, Protection and Control, pp. 353–385. Edited by B. R. Bloom. Washington, DC: American Society for Microbiology.
  35. Winder, F. G. & Rooney, S. A. ( 1970; ). Effects of nitrogenous components of the medium on the carbohydrate and nucleic acid content of Mycobacterium tuberculosis BCG. J Gen Microbiol 63, 29–39.[CrossRef]
    [Google Scholar]
  36. Woldringh, C. L., Binnerts, J. S. & Mans, A. ( 1981; ). Variation in Escherichia coli buoyant density measured in percoll gradients. J Bacteriol 148, 58–63.
    [Google Scholar]
  37. Yoshimura, F. & Nikaido, H. ( 1982; ). Permeability of Pseudomonas aeruginosa outer membrane to hydrophobic solutes. J Bacteriol 152, 636–642.
    [Google Scholar]
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