1887

Abstract

In order to further understand the different physiological states of the tubercle bacillus, a frame of reference was sought by first correlating the macromolecular compositions of with specific growth rates and also with the rates of protein synthesis. Data for DNA : protein : RNA were converted to the average amounts of DNA [ ], protein [ ] and RNA [ ] per cell. The specific growth rate was found to be directly proportional to / . The specific protein synthesis rate per average cell [ ] was shown to be directly proportional to the third power of the ratio / which reflects the ribosome concentration. The equations derived were shown apply to both (=1·73 h) and BCG (=0·029 h).

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.25645-0
2003-03-01
2020-08-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/149/3/mic149729.html?itemId=/content/journal/micro/10.1099/mic.0.25645-0&mimeType=html&fmt=ahah

References

  1. Al-Karadagh S., Kristensen O., Liljas A.. 2000; A decade of progress in understanding the structural basis of protein synthesis. Prog Biophys Mol Biol73:167–193
    [Google Scholar]
  2. Armstrong J. A., D'Arcy-Hart P.. 1971; Response of cultured macrophages to Mycobacterium tuberculosis , with observations on fusion of lysosomes with phagosomes. J Exp Med134:713–740
    [Google Scholar]
  3. Arnstein H. R. V., Cox R. A.. 1992; Protein Biosynthesis: in Focus Oxford: Oxford University Press;
    [Google Scholar]
  4. Baldwin W. W., Hirkish M. A., Koch A. L.. 1994; A change in a single gene of Salmonella typhimurium can dramatically change its buoyant density. J Bacteriol176:5001–5004
    [Google Scholar]
  5. Bremer H., Dennis P. P.. others 1996; Modulation of chemical composition and other parameters of the cell growth rate. In Escherichia coli and Salmonella: Cellular and Molecular Biology , 2nd edn. pp 1553–1568 Editor by Neidhardt F. C.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Brosch R., Gordon S. V., Eiglmeier K., Garnier T., Tekaia F., Yeramian E., Cole S. T.. 2000; Genomics, biology, and evolution of the Mycobacterium tuberculosis complex. In Molecular Genetics of Mycobacteria pp 19–36 Edited by Hatfull G. F., Jacobs W. R. Jr. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Butcher P. O., Sole K. M., Mangan J. A.. 1999; RNA extraction. In Molecular Mycobacteriology: Techniques and Clinical Applications pp 385–350 Edited by Ollar R. A., Connell N. O.. New York: Marcel Dekker;
    [Google Scholar]
  8. Cole S. T., Brosch R., Parkhill J.. 39 other authors 1998; Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature393:537–544
    [Google Scholar]
  9. Colston M. J., Cox R. A.. 1999; Mycobacterial growth and dormancy. In Mycobacteria: Molecular Biology and Virulence pp 198–219 Edited by Ratledge C., Dale J.. Oxford, UK: Blackwell Science;
    [Google Scholar]
  10. Cooper S.. 1988; Rate and topography of cell wall synthesis during the division cycle of Salmonella typhimurium . J Bacteriol170:422–430
    [Google Scholar]
  11. Cooper S., Helmstetter C. E.. 1968; Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol31:519–540
    [Google Scholar]
  12. Diaper J. P., Edwards C.. 1994; Survival of Staphylococcus aureus in lakewater monitored by flow cytometry. Microbiology140:35–42
    [Google Scholar]
  13. Ferrari G., Langen H., Naito M., Pieters J.. 1999; A coat protein on phagosomes involved in the intracellular survival of mycobacteria. Cell97:435–447
    [Google Scholar]
  14. Flärdh K., Cohen P. S., Kellenberg S.. 1992; Ribosomes exist in large excess over the apparent demand for protein synthesis during carbon starvation in marine Vibrio sp. strain CCUG 15956. J Bacteriol174:6780–6788
    [Google Scholar]
  15. Gonzalez-y-Merchand J. A., Colston M. J., Cox R. A.. 1999; Effects of growth conditions on expression of mycobacterial mur A and tyr S genes and contributions of their transcripts to precursor rRNA synthesis. J Bacteriol181:4617–4627
    [Google Scholar]
  16. Helmstetter C. E., Cooper S.. 1968; DNA synthesis during the division cycle of rapidly growing E. coli B/r. J Mol Biol31:507–518
    [Google Scholar]
  17. Hiriyanna K. T., Ramakrishnan T.. 1986; Deoxyribonucleic acid replication time in Mycobacterium tuberculosis H37 Rv. Arch Microbiol144:105–109
    [Google Scholar]
  18. Ingraham J. L., Maaløe O., Neidhardt F. C.. 1983; Growth of the Bacterial Cell Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  19. Jacobs W. R. Jr. 2000; Mycobacterium tuberculosis : a once genetically intractable organism. In Molecular Genetics of Mycobacteria pp 1–16 Edited by Hatfull G. F., Jacobs W. R. Jr. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Jacobsen H.. 1974; PhD thesis University of Copenhagen;
  21. Koch A. L.. 1979; Microbial growth in low concentrations of nutrients. In Strategies of Microbial Life in Extreme Environments pp 261–269 Edited by Shilo M.. Weinheim: Verlag Chemie;
    [Google Scholar]
  22. Maaløe O., Kjeldgaard N. O.. 1966; Control of Macromolecular Synthesis: a Study of DNA, RNA and Protein Synthesis in Bacteria New York: W. A. Benjamin;
    [Google Scholar]
  23. Pape T., Wintermeyer W., Rodnina M. V.. 1998; Complete kinetic mechanism of elongation factor Tu-dependent binding of aminoacyl-tRNA to the A-site of the E. coli ribosome. EMBO J17:7490–7497
    [Google Scholar]
  24. Pedersen S., Bloch P. L., Reeh S., Neidhardt F. C.. 1978; Patterns of protein synthesis in E. coli : a catalog of the amount of 140 individual proteins at different growth rates. Cell14:179–190
    [Google Scholar]
  25. Powell E. O.. 1956; Growth rate and generation time of bacteria, with special reference to continuous culture. J Gen Microbiol15:492–511
    [Google Scholar]
  26. Ratledge C.. 1982; Nutrition, growth and metabolism. In Biology of the Mycobacteria pp 185–271 Edited by Ratledge C., Stanford J. L.. London: Academic Press;
    [Google Scholar]
  27. Schaechter M., Williamson J. P., Hood J. R. Jr, Koch A. L.. 1962; Growth, cell and nuclear divisions in some bacteria. J Gen Microbiol29:421–434
    [Google Scholar]
  28. Shepard C. C.. 1960; The experimental disease that follows the injection of human leprosy bacilli into the footpads of mice. J Exp Med112:445–454
    [Google Scholar]
  29. Turner K., Porter J., Pickup R., Edwards C.. 2000; Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry. J Appl Microbiol89:90–99
    [Google Scholar]
  30. Wayne L. G.. 1994; Cultivation of Mycobacterium tuberculosis for research purposes. In Tuberculosis: Pathogenesis, Protection and Control pp 73–83 Edited by Bloom B.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  31. Wayne L. G., Hayes L. G.. 1996; An in vitro model for sequential study of shift down of Mycobacterium tuberculosis through two stages of replicating persistence. Infect Immun64:2062–2069
    [Google Scholar]
  32. Wayne L. G., Kubica G. P.. 1986; The mycobacteria. In Bergey's Manual of Systematic Bacteriology vol. 2 pp 1435–1457 Edited by A P. H., Sneath N. S., Mair M. E. Sharpe., Holt J. G.. Baltimore: Williams & Wilkins;
    [Google Scholar]
  33. Weber I., Fritz C., Ruttkowski S., Kreft A., Bange F. C.. 2000; Anaerobic nitrate reductase ( narGHJI ) activity of Mycobacterium bovis BCG in vitro and its contribution to virulence in immunodeficient mice. Mol Microbiol35:1017–1025
    [Google Scholar]
  34. Wheeler P. R., Ratledge C.. 1994; Metabolism of Mycobacterium tuberculosis . In Tuberculosis; Pathogenesis, Protection and Control pp 353–385 Edited by Bloom B. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  35. Winder F. G., Rooney S. A.. 1970; Effects of nitrogenous components of the medium on the carbohydrate and nucleic acid content of Mycobacterium tuberculosis BCG. J Gen Microbiol63:29–39
    [Google Scholar]
  36. Woldringh C. L., Binnerts J. S., Mans A.. 1981; Variation in Escherichia coli buoyant density measured in percoll gradients. J Bacteriol148:58–63
    [Google Scholar]
  37. Yoshimura F., Nikaido H.. 1982; Permeability of Pseudomonas aeruginosa outer membrane to hydrophobic solutes. J Bacteriol152:636–642
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.25645-0
Loading
/content/journal/micro/10.1099/mic.0.25645-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error