1887

Abstract

Arabinan polysaccharide side-chains are present in both and in the heteropolysaccharide arabinogalactan (AG), and in in the lipoglycan lipoarabinomannan (LAM). This study shows by quantitative sugar and glycosyl linkage analysis that possesses a much smaller LAM version, Cg-LAM, characterized by single -Ara residues linked to the (1→6)-linked mannan backbone. MALDI-TOF MS showed an average molecular mass of 13 800–15 400 Da for Cg-LAM. The biosynthetic origin of Ara residues found in the extracytoplasmic arabinan domain of AG and LAM is well known to be provided by decaprenyl-monophosphoryl arabinose (DPA). However, the characterization of LAM in a  : :  mutant devoid of prenyltransferase activity and devoid of DPA-dependent arabinan deposition into AG revealed partial formation of LAM, albeit with a slightly altered molecular mass. These data suggest that in addition to DPA utilization as an Ara donor, alternative pathways exist in for Ara delivery, possibly via an unknown sugar nucleotide.

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2007-08-01
2019-10-19
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