1887

Abstract

MbtH-like proteins are a family of small proteins encoded by genes found in many, but not all, non-ribosomal peptide synthetase-encoding gene clusters that direct the biosynthesis of peptide antibiotics and siderophores. Studies published to date have not elucidated the function of MbtH-like proteins, nor have they clarified whether they are required for metabolite biosynthesis. Here it is shown that only one of two genes ( or ) encoding MbtH-like proteins in is required for biosynthesis of the peptide siderophore coelichelin and the calcium-dependent peptide antibiotic (CDA). The and genes can functionally complement each other , suggesting that CdaX and CchK can cross-talk with the coelichelin and CDA biosynthetic pathways, respectively. Transcriptional analyses of wild-type and a double replacement mutant indicate that CchK and CdaX may not be involved in transcriptional regulation of coelichelin and CDA biosynthetic gene clusters.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.2006/003145-0
2007-05-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/153/5/1405.html?itemId=/content/journal/micro/10.1099/mic.0.2006/003145-0&mimeType=html&fmt=ahah

References

  1. Barona-Gómez F., Wong U., Giannakopulos A. E., Derrick P. J., Challis G. L. 2004; Identification of a cluster of genes that directs desferrioxamine biosynthesis in Streptomyces coelicolor M145. J Am Chem Soc 126:16282–16283 [CrossRef]
    [Google Scholar]
  2. Barona-Gómez F., Lautru S., Francou F.-X., Pernodet J.-L., Leblond P., Challis G. L. 2006; Multiple biosynthetic and uptake systems mediate siderophore-dependent iron acquisition in Streptomyces coelicolor A3(2) and Streptomyces ambofaciens ATCC 23877. Microbiology 152:3355–3366 [CrossRef]
    [Google Scholar]
  3. Bate N., Bignell D. R. D., Cundliffe E. 2006; Regulation of tylosin biosynthesis involving ‘SARP-helper’ activity. Mol Microbiol 62:148–156 [CrossRef]
    [Google Scholar]
  4. Carter R. A., Worsley P. S., Sawers G., Challis G. L., Dilworth M. J., Carson K. C., Lawrence J. A., Wexler M., Johnston A. W. B., Yeoman K. H. 2002; The vbs genes that direct synthesis of the siderophore vicibactin in Rhizobium leguminosarum : their expression in other genera requires ECF sigma factor RpoI. Mol Microbiol 44:1153–1166 [CrossRef]
    [Google Scholar]
  5. Cole S. T., Brosch R., Parkhill J., Garnier T., Churcher C., Harris D., Gordon S. V., Eiglmeier K., Gas S. other authors 1998; Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537–544 [CrossRef]
    [Google Scholar]
  6. Datsenko K. A., Wanner B. L. 2000; One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97:6640–6645 [CrossRef]
    [Google Scholar]
  7. Doumith M., Weingarten P., Wehmeier U. F., Salah-Bey K., Benhamou C., Capdevila C., Michel J. M., Piepersberg W., Raynal M. C. 2000; Analysis of genes involved in 6-deoxyhexose biosynthesis and transfer in Saccharopolyspora erythraea. Mol Gen Genet 264:477–485 [CrossRef]
    [Google Scholar]
  8. Du L., Chen M., Sanchez C., Shen B. 2000; An oxidation domain in the BlmIII non-ribosomal peptide synthetase probably catalyzing thiazole formation in the biosynthesis of the anti-tumor drug bleomycin in Streptomyces verticillus ATCC 15003. FEMS Microbiol Lett 189:171–175 [CrossRef]
    [Google Scholar]
  9. Edwards D. J., Gerwick W. H. 2004; Lyngbyatoxin biosynthesis: sequence of biosynthetic gene cluster and identification of a novel aromatic prenyltransferase. J Am Chem Soc 126:11432–11433 [CrossRef]
    [Google Scholar]
  10. Gehring A. M., Mori I., Walsh C. T. 1998; Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF. Biochemistry 37:2648–2659 [CrossRef]
    [Google Scholar]
  11. Gust B., Chandra G., Jakimowicz D., Yuqing T., Bruton C. J., Chater K. F. 2004; λ Red-mediated genetic manipulation of antibiotic-producing Streptomyces. Adv Appl Microbiol 54:107–128
    [Google Scholar]
  12. Hojati Z., Milne C., Harvey B., Gordon L., Borg M., Flett F., Wilkinson B., Sidebottom P. J., Rudd B. A. other authors 2002; Structure, biosynthetic origin, and engineered biosynthesis of calcium-dependent antibiotics from Streptomyces coelicolor. Chem Biol 9:1175–1187 [CrossRef]
    [Google Scholar]
  13. Kieser T., Bibb J. M., Buttner M. J., Chater K. F., Hopwood D. A. 2000 Practical Streptomyces Genetics Norwich: The John Innes Foundation;
    [Google Scholar]
  14. Lauer B., Russwurm R., Bormann C. 2000; Molecular characterization of two genes from Streptomyces tendae Tu901 required for the formation of the 4-formyl-4-imidazolin-2-one-containing nucleoside moiety of the peptidyl nucleoside antibiotic nikkomycin. Eur J Biochem 267:1698–1706 [CrossRef]
    [Google Scholar]
  15. Lautru S., Deeth R. J., Bailey L., Challis G. L. 2005; Discovery of a new peptide natural product by Streptomyces coelicolor genome mining. Nat Chem Biol 1:265–269 [CrossRef]
    [Google Scholar]
  16. Müller G., Raymond K. N. 1984; Specificity and mechanism of ferrioxamine-mediated iron transport in Streptomyces pilosus. J Bacteriol 160:304–312
    [Google Scholar]
  17. Oh E. T., So J.-S. 2003; A rapid method for RNA preparation from Gram-positive bacteria. J Microbiol Methods 52:395–398 [CrossRef]
    [Google Scholar]
  18. Pettis G. S., McIntosh M. A. 1987; Molecular characterization of the Escherichia coli enterobactin cistron entF and coupled expression of entF and the fes gene. J Bacteriol 169:4154–4162
    [Google Scholar]
  19. Quadri L. E. N., Sello J., Keating T. A., Weinreb P. H., Walsh C. T. 1998; Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for the assembly of the virulence-conferring siderophore mycobactin. Chem Biol 5:631–645 [CrossRef]
    [Google Scholar]
  20. Sambrook J., Russell D. W. 2001 Molecular Cloning: a Laboratory Manual , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Soliveri J. A., Gomez J., Bishai W. R., Chater K. F. 2000; Multiple paralogous genes related to the Streptomyces coelicolor developmental regulatory gene whiB are present in Streptomyces and other actinomycetes. Microbiology 146:333–343
    [Google Scholar]
  22. Sondén B., Kocíncová D., Deshayes C., Euphrasie D., Thayat L., Laval F., Frehel C., Etienne G., Reyrat J.-M., Daffé M. 2005; Gap, a mycobacterial specific integral membrane protein, is required for glycolipid transport to the cell surface. Mol Microbiol 58:426–440 [CrossRef]
    [Google Scholar]
  23. Stegmann E., Rausch C., Stockert S., Burkert D., Wohlleben W. 2006; The small MbtH-like protein encoded by an internal gene of the balhimycin biosynthetic gene cluster is not required for glycopeptide production. FEMS Microbiol Lett 262:85–92 [CrossRef]
    [Google Scholar]
  24. Takano E., White J., Thompson C. J., Bibb M. J. 1995; Construction of thiostrepton-inducible, high-copy-number expression vectors for use in Streptomyces spp. Gene 166:133–137 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.2006/003145-0
Loading
/content/journal/micro/10.1099/mic.0.2006/003145-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error