1887

Abstract

The bacterium , a fish pathogen, uses the type three secretion system (TTSS) to inject effector proteins into host cells to promote the infection. The study of the genome of has revealed the existence of Ati2, a potential TTSS effector protein. In the present study, a structure–function analysis of Ati2 has been done to determine its role in the virulence of . Biochemical assays revealed that Ati2 is secreted into the medium in a TTSS-dependent manner. Protein sequence analyses, molecular modelling and biochemical assays demonstrated that Ati2 is an inositol polyphosphate 5-phosphatase, which hydrolyses PtdIns(4,5)P and PtdIns(3,4,5)P in a way similar to VPA0450, a protein from having high sequence similarity with Ati2. Mutants of Ati2 with altered amino acids at two different locations in the catalytic site displayed no phosphatase activity. Wild-type and mutant forms of Ati2 were cloned into expression systems for , a soil amoeba used as an alternative host to study virulence. Expression tests allowed us to demonstrate that Ati2 is toxic for the host cell in a catalytic-dependent manner. Finally, this study demonstrated the existence of a new TTSS effector protein in .

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2013-09-01
2022-10-01
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