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Volume 157, Issue 7

Identification of the region and its influence on heterocyst development in the filamentous cyanobacterium sp. strain PCC 7120 Free

Abstract

sp. strain PCC 7120 ( PCC 7120) is a filamentous, nitrogen-fixing cyanobacterium. Upon deprivation of combined nitrogen, about 5–10 % of the cells become heterocysts, i.e. cells devoted to N fixation. Heterocysts are intercalated among vegetative cells and distributed in a semi-regular pattern, and adjacent heterocysts are rarely observed. Previously, we showed that the cell cycle could play a regulatory function during heterocyst development, although the mechanism involved remains unknown. As a further step to understand this phenomenon, we identified the region for chromosomal DNA replication, located between and . The region of PCC 7120 was able to support the self-replication of a plasmid in the unicellular cyanobacterium sp. PCC 6803. Surprisingly, integration of the region into the chromosome of PCC 7120 through homologous recombination led to much slower cell growth in the absence of a combined-nitrogen source and to multiple contiguous proheterocysts after prolonged incubation. Real-time RT-PCR showed that expression of two heterocyst-related genes, and , was altered in these strains: expression remained high 48 h after induction, and increased to high levels after induction for 12 h. These results suggest that the balance between and DnaA could be important for heterocyst development.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 30970088 and 30670046)
© 2011 SGM
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2011-07-01
2024-04-26
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Identification of the oriC region and its influence on heterocyst development in the filamentous cyanobacterium Anabaena sp. strain PCC 7120
Microbiology 157, 1910 (2011); https://doi.org/10.1099/mic.0.047241-0
/content/journal/micro/10.1099/mic.0.047241-0
/content/journal/micro/10.1099/mic.0.047241-0
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