1887

Abstract

A sporulated lactic acid bacterium (LAB) isolated from cider must was shown to harbour the gene encoding tyrosine decarboxylase. The isolate belonged to the genus and may correspond to a novel species. The ability of the -positive strain, sp. strain P3J, to produce tyramine was demonstrated by using HPLC. A 7535 bp nucleotide sequence harbouring the putative gene was determined. Analysis of the obtained sequence showed that four tyramine production-associated genes [tyrosyl-tRNA synthetase (), tyrosine decarboxylase (), tyrosine permease () and Na/H antiporter ()] were present and were organized as already described in other tyramine-producing LAB. This operon was surrounded by genes showing the highest identities with mobile elements: a putative phage terminase and a putative transposase (downstream and upstream, respectively), suggesting that the tyramine-forming trait was acquired through horizontal gene transfer. Transcription analyses of the gene cluster suggested that and are expressed as monocistronic genes while would be part of a polycistronic mRNA together with . The presence of tyrosine in the culture medium induced the expression of all genes except for A clear correlation was observed between initial tyrosine concentration and tyramine production combined with an increase in the final pH reached by the culture. Finally, cloning and expression of the gene in demonstrated that its product catalyses the exchange of tyrosine and tyramine.

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2011-06-01
2019-12-07
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