1887

Abstract

Under stressful conditions in nature, forms biofilms for long-term survival. Curli fimbriae are an essential architecture for cell–cell contacts within biofilms. Structural components and assembly factors of curli are encoded by two operons, and . The gene product controls transcription of both operons. Reflecting the response of expression to external stresses, a number of transcription factors participate in the regulation of the promoter. Analysis of the mRNA obtained from mutants in different transcription factors indicated that CpxR and H-NS act as repressors while OmpR, RstA and IHF act as activators. An acid-stress response regulator, RstA, activates only under acidic conditions. These five factors bind within a narrow region of about 200 bp upstream of the promoter. After pair-wise promoter-binding assays, the increase in transcription in the stationary phase was suggested to be due, at least in part, to the increase in IHF level cancelling the silencing effect of H-NS. In addition, we propose a novel regulation model of this complex promoter through cooperation between the two positive factors (OmpR–IHF and RstA–IHF) and also between the two negative factors (CpxR–H-NS).

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2010-08-01
2019-12-08
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