1887

Abstract

Aerobic enrichments from soda lake sediments with CO as the only substrate resulted in the isolation of five bacterial strains capable of autotrophic growth with CO at extremely high pH and salinity. The strains belonged to the / cluster in the Gammaproteobacteria, where the ability to oxidize CO, but not growth with CO, has been demonstrated previously. The growth with CO was possible only at an oxygen concentration below 5 % and CO concentration below 20 % in the gas phase. The isolates were also capable of growth with formate but not with H. The carboxydotrophic growth occurred within a narrow pH range from 8 to 10.5 (optimum at 9.5) and a broad salt concentration from 0.3 to 3.5 M total Na (optimum at 1.0 M). Cells grown on CO had high respiration activity with CO and formate, while the cells grown on formate actively oxidized formate alone. In CO-grown cells, CO-dehydrogenase (CODH) activity was detectable both in soluble and membrane fractions, while the NAD-independent formate dehydrogenase (FDH) resided solely in membranes. The results of total protein profiling and the failure to detect CODH with conventional primers for the gene indicated that the CO-oxidizing enzyme in haloalkaliphilic isolates might differ from the classical aerobic CODH complex. A single gene encoding the RuBisCO large subunit was detected in all strains, suggesting the presence of the Calvin cycle of inorganic carbon fixation. Overall, these results demonstrated the possibility of aerobic carboxydotrophy under extremely haloalkaline conditions.

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2010-03-01
2019-10-22
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References

  1. De Ley, J., Caffon, H. & Reinaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturationrates. Eur J Biochem 12, 133–140.[CrossRef]
    [Google Scholar]
  2. Dunfield, K. E. & King, G. M. ( 2004; ). Molecular analysis of carbon monoxide-oxidizing bacteria associated withrecent Hawaiian volcanic deposits. Appl Environ Microbiol 70, 4242–4248.[CrossRef]
    [Google Scholar]
  3. Hardy, K. R. & King, G. M. ( 2001; ).Enrichment of high-affinity CO oxidizers in Maine forest soil. Appl Environ Microbiol 67, 3671–3676.[CrossRef]
    [Google Scholar]
  4. Hoeft, S. E., Switzer Blum, J., Stolz, J. F., Tabita, F. R.,Witte, B., King, G. M., Santini, J. M. & Oremland, R. S. ( 2007; ). Alkalilimnicola ehrlichii sp. nov., a novel arsenite-oxidizing,haloalkaliphilic gammaproteobacterium capable of chemoautotrophic or heterotrophicgrowth with nitrate or oxygen as the electron acceptor. Int J SystEvol Microbiol 57, 504–512.
    [Google Scholar]
  5. Hoehler, T. M., Bebout, B. M. & Des Marais, D. J. ( 2001; ). The role of microbial mats in the production of reducedgases on the early Earth. Nature 412, 324–327.[CrossRef]
    [Google Scholar]
  6. King, G. M. ( 2003; ). Molecular and culture-basedanalyses of aerobic carbon monoxide oxidizer diversity. Appl EnvironMicrobiol 69, 7257–7265.
    [Google Scholar]
  7. King, G. M. & Weber, C. F. ( 2007; ).Distribution, diversity and ecology of aerobic CO-oxidizing bacteria. Nat Rev Microbiol 5, 107–118.[CrossRef]
    [Google Scholar]
  8. Laemmli, U. K. ( 1970; ). Cleavage of structuralproteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  9. Lorite, M. J., Tachil, J., Sanjua, J. N., Meyer, O. & Berdmar,E. J. ( 2000; ). Carbon monoxide dehydrogenase activityin Bradyrhizobium japonicum. Appl Environ Microbiol 66, 1871–1876.[CrossRef]
    [Google Scholar]
  10. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall,R. J. ( 1951; ). Protein measurement with Folin phenolreagent. J Biol Chem 193, 265–275.
    [Google Scholar]
  11. Marmur, J. ( 1961; ). A procedure for theisolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  12. Marmur, J. & Doty, P. ( 1962; ). Determinationof the base composition of deoxyribonucleic acid from microorganisms. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  13. Meyer, O. & Schlegel, H. G. ( 1978; ). Reisolation of the carbon monoxide utilizing hydrogen bacterium Pseudomonas carboxydovorans (Kistner) comb. nov. Arch Microbiol 118, 35–43.[CrossRef]
    [Google Scholar]
  14. Meyer, O., Frunzke, K., Gadkari, D., Jacobitz, S., Hugendieck,I. & Kraut, M. ( 1990; ). Utilization of carbon monoxideby aerobes: recent advances. FEMS Microbiol Rev 87, 253–260.[CrossRef]
    [Google Scholar]
  15. Mörsdorf, G., Frunzke, K., Gadkari, D. & Meyer, O. ( 1992; ). Microbial growth on carbon monoxide. Biodegradation 3, 61–82.
    [Google Scholar]
  16. Oremland, R. S., Hoeft, S. E., Santini, J. M., Bano, N., Hollibaugh,R. A. & Hollibaugh, J. T. ( 2002; ). Anaerobic oxidationof arsenite in Mono Lake water and by a facultative, arsenite-oxidizing chemoautotroph,strain MLHE-1. Appl Environ Microbiol 68, 4795–4802.[CrossRef]
    [Google Scholar]
  17. Pfennig, N. & Lippert, K. D. ( 1966; ). Über das Vitamin B12-bedürfnis phototropher Schwefelbacterien. Arch Microbiol 55, 245–256.
    [Google Scholar]
  18. Schäfer, H. & Muyzer, G. ( 2001; ). Denaturing gradient gel electrophoresis in marine microbial ecology. Methods Microbiol 30, 425–468.
    [Google Scholar]
  19. Sorokin, D. Y. & Kuenen, J. G. ( 2005; ). Alkaliphilic chemolithotrophs from soda lakes. FEMS MicrobiolEcol 52, 287–295.
    [Google Scholar]
  20. Sorokin, D. Y., Banciu, H., Robertson, L. A. & Kuenen, J.G. ( 2006a; ). Haloalkaliphilic sulfur-oxidizing bacteria.In: The Prokaryotes, Ecophysiology and Biochemistry, vol. 2, pp. 969–984. Edited by M. Dworkin, S. Falkow, E. Rosenberg,K. H. Schleifer & E. Stackebrandt. New York: Springer.
  21. Sorokin, D. Y., Zhilina, T. N., Lysenko, A. M., Tourova, T.P. & Spiridonova, E. M. ( 2006b; ). Metabolic versatilityof haloalkaliphilic bacteria from soda lakes belonging to the Alkalispirillum-Alkalilimnicola group. Extremophiles 10, 213–220.[CrossRef]
    [Google Scholar]
  22. Spiridonova, E. M., Berg, I. A., Kolganova, T. V., Ivanovsky,R. N., Kuznetsov, B. B. & Tourova, T. P. ( 2004; ).An oligonucleotide primer system for amplification of the ribulose-1,5-bisphosphatecarboxylase/oxygenase genes of bacteria of various taxonomic groups. Microbiology 73, 316–325.[CrossRef]
    [Google Scholar]
  23. Tolli, J. D., Sievert, S. M. & Taylor, C. D. ( 2006; ). Unexpected diversity of bacteria capable of carbon monoxideoxidation in a coastal marine environment, and contribution of the Roseobacter-associated clade to total CO oxidation. Appl Environ Microbiol 72, 1966–1973.[CrossRef]
    [Google Scholar]
  24. Tourova, T. P., Spiridonova, E. M., Berg, I. A., Slobodova,N. V., Boulygina, E. S. & Sorokin, D. Y. ( 2007; ).Phylogeny and evolution of the family Ectothiorhodospiraceae basedon comparison of 16S rRNA, cbbL and nifH genes. Int J Syst Evol Microbiol 57, 2387–2398.[CrossRef]
    [Google Scholar]
  25. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the constructionand drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
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Cell morphology of the CO-utilizing isolates from soda lakes grown with CO at pH 10 [PDF](651 KB)

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Reduced minus air-oxidized cytochrome spectra of strain ACO1 grown with CO [PDF](556 KB)

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