1887

Abstract

complex (MAC) currently comprises eight species of environmental and animal-associated, slowly-growing mycobacteria: , , , , , , and . In humans, MAC organisms are responsible for opportunistic infections whose unique epidemiology remains poorly understood, in part due to the lack of a genotyping method applicable to all eight MAC species. In this study we developed multispacer sequence typing (MST), a sequencing-based method, for the genotyping of MAC organisms. An alignment of the genome sequence of subsp. strain 104 and subsp. strain K-10 revealed 621 intergenic spacers <1000 bp. From these, 16 spacers were selected that ranged from 300 to 800 bp and contained a number of variable bases, <50 within each of the 16 spacers. Four spacers were successfully PCR-amplified and sequenced in 11 reference strains. Combining the sequence of these four spacers in 106 MAC organisms, including 83 , 11 , six , two and one each of , , and , yielded a total of 45 spacer types, with an index of discrimination of 0.94. Each spacer type was specific for a species and certain spacer types were specific for subspecies of . MST is a new method for genotyping of organisms belonging to any one of the eight MAC species tested in this study.

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2010-03-01
2019-11-19
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Strains and clinical isolates from the clinical microbiology laboratory of La Timone Hospital used in this study [ PDF] (21 kb) Results of RFLP IS genotyping. Pictures of RFLP IS assay membranes. MAA, subsp. ; MAH, subsp. [ PDF] (434 kb) Results of RFLP IS genotyping [ PDF] (102 kb)

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Strains and clinical isolates from the clinical microbiology laboratory of La Timone Hospital used in this study [ PDF] (21 kb) Results of RFLP IS genotyping. Pictures of RFLP IS assay membranes. MAA, subsp. ; MAH, subsp. [ PDF] (434 kb) Results of RFLP IS genotyping [ PDF] (102 kb)

PDF

Strains and clinical isolates from the clinical microbiology laboratory of La Timone Hospital used in this study [ PDF] (21 kb) Results of RFLP IS genotyping. Pictures of RFLP IS assay membranes. MAA, subsp. ; MAH, subsp. [ PDF] (434 kb) Results of RFLP IS genotyping [ PDF] (102 kb)

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