1887

Abstract

Bacteria growing as surface-adherent biofilms are better able to withstand chemical and physical stresses than their unattached, planktonic counterparts. Using transcriptional profiling and quantitative PCR, we observed a previously uncharacterized gene, to be upregulated during MG1655 biofilm growth in a chemostat on serine-limited defined medium. A mutant, developed through targeted-insertion mutagenesis, and a -complemented strain, were obtained for further characterization. While bacterial surface colonization levels (c.f.u. cm) were similar in all three strains, the mutant strain exhibited reduced microcolony formation when observed in flow cells, and greatly enhanced flagellar motility on soft (0.3 %) agar. Complementation of restored microcolony formation and flagellar motility to wild-type levels. Cell surface hydrophobicity and twitching motility were unaffected by the presence or absence of . In contrast to the parent strain, biofilms from the mutant strain were less able to resist acid and peroxide stresses. had no significant effect on biofilm susceptibility to alkali or heat stress. Planktonic cultures from all three strains showed similar responses to these stresses. Regardless of the presence of , planktonic withstood alkali stress better than biofilm populations. Complementation of restored viability following exposure to peroxide stress, but did not restore acid resistance. Based on its influence on biofilm maturation and stress response, and effects on motility, we propose renaming the uncharacterized gene, , as (iofilm tress and otility).

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2010-01-01
2019-10-19
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