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Volume 155, Issue 5

A permease encoded by is required for active glycerol uptake by Free

Abstract

accumulates large amounts of the polyols glycerol and -arabitol when the cells are exposed to physiological conditions relevant to stress and virulence in animals. Intracellular concentrations of glycerol are determined by rates of glycerol production and catabolism and of glycerol uptake and efflux through the plasma membrane. We and others have studied glycerol production in , but glycerol uptake by has not been studied. In the present study, we found that [C]glycerol uptake by SC5314 was (i) accumulative; (ii) dependent on proton-motive force; (iii) unaffected by carbon source; and (iv) unaffected by large molar excesses of -arabitol or other polyols. The respective and values were 2.1 mM and 460 μmol h (g dry wt) in glucose medium and 2.6 mM and 268 μmol h (g dry wt) in glycerol medium. To identify the glycerol uptake protein(s), we cloned the homologues of the genes and , both of which are known to be involved in glycerol transport. When multicopy plasmids encoding , and were introduced into the corresponding null mutants, the transformants all acquired the ability to grow on minimal glycerol medium; however, only null mutants transformed with actively took up extracellular [C]glycerol. When both chromosomal alleles of were deleted from BWP17, the resulting null mutants grew poorly on minimal glycerol medium, and their ability to transport [C]glycerol into the cell was markedly reduced. In contrast, deletion of both chromosomal alleles of or of had no significant effects on [C]glycerol uptake or the ability to grow on minimal glycerol medium. Northern blot analysis indicated that was expressed in both glucose and glycerol media, conditions under which we detected wild-type active glycerol uptake. Furthermore, was highly expressed in salt-stressed cells; however, the null mutant was no more sensitive to salt stress than wild-type controls. We also detected high levels of expression in glycerol-grown cells, even though deletion of this gene did not influence glycerol uptake activity in glycerol-grown cells. We conclude from the results above that a plasma-membrane H symporter encoded by actively transports glycerol into cells.

  • Received:
  • Accepted:
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Keyword(s): CCCP, carbonyl cyanide m-chlorophenylhydrazone
SGM
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2009-05-01
2024-04-26
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A permease encoded by STL1 is required for active glycerol uptake by Candida albicans
Microbiology 155, 1547 (2009); https://doi.org/10.1099/mic.0.023457-0
/content/journal/micro/10.1099/mic.0.023457-0
/content/journal/micro/10.1099/mic.0.023457-0
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