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Volume 167, Issue 12

The genetic basis of phage susceptibility, cross-resistance and host-range in Open Access

Abstract

Though bacteriophages (phages) are known to play a crucial role in bacterial fitness and virulence, our knowledge about the genetic basis of their interaction, cross-resistance and host-range is sparse. Here, we employed genome-wide screens in serovar Typhimurium to discover host determinants involved in resistance to eleven diverse lytic phages including four new phages isolated from a therapeutic phage cocktail. We uncovered 301 diverse host factors essential in phage infection, many of which are shared between multiple phages demonstrating potential cross-resistance mechanisms. We validate many of these novel findings and uncover the intricate interplay between RpoS, the virulence-associated general stress response sigma factor and RpoN, the nitrogen starvation sigma factor in phage cross-resistance. Finally, the infectivity pattern of eleven phages across a panel of 23 genome sequenced strains indicates that additional constraints and interactions beyond the host factors uncovered here define the phage host range.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genetic screen , genome-wide , host-range , phage , resistance , salmonella , sigma factor and virulence
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2021-12-15
2024-04-26
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.001126
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The genetic basis of phage susceptibility, cross-resistance and host-range in Salmonella
Microbiology 167, 001126 (2021); https://doi.org/10.1099/mic.0.001126
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