1887

Abstract

WhiB is a transcription regulator which has been reported to be involved in the regulation of cell morphogenesis, cell division, antibiotic resistance, stress, etc., in several members of the family . The present study describes functional characterization of a WhiB family protein, WhiB1 (protein ID: WP_065632651.1), from sp. IITR100. We demonstrate that WhiB1 affects chromosome segregation and cell morphology in recombinant , sp. IITR100 as well as in . Multiple sequence alignment suggests that WhiB1 is a conserved protein among members of the family . It has been reported that overexpression of WhiB1 leads to repression of the biodesulfurization operon in recombinant , sp. IITR100 and . A WhiB1-mut containing a point mutation Q116A in the DNA binding domain of WhiB1 led to partial alleviation of repression of the biodesulfurization operon. We show for the first time that the WhiB family protein WhiB1 is also involved in repression of the biodesulfurization operon by directly binding to the promoter DNA.

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/content/journal/micro/10.1099/mic.0.000985
2020-11-20
2020-12-04
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