1887

Abstract

The thermophilic and are able to use citrate or C-dicarboxylates like fumarate or succinate as the substrates for growth. The genomes of the sequenced strains (nine strains) each encoded a two-component system of the CitA family. The sensor kinase of (termed CitA) was able to replace CitA of (CitA) in a heterologous complementation assay restoring expression of the CitA-dependent reporter gene and anaerobic growth on citrate. Complementation was specific for citrate. The sensor kinase of (termed DcuS) was able to replace DcuS of . It responded in the heterologous expression system to C-dicarboxylates and to citrate, suggesting that DcuS is, like DcuS, a C-dicarboxylate sensor with a side-activity for citrate. DcuS, unlike the homologous DctS from , required no binding protein for function in the complementation assay. Thus, the thermophilic and contain citrate and C-dicarboxylate sensor kinases of the CitA and DcuS type, respectively, and retain function and substrate specificity under mesophilic growth conditions in .

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2016-01-01
2024-12-14
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