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Volume 161, Issue 11

The metabolism of ()-3-hydroxybutyrate is regulated by the enhancer-binding protein PA2005 and the alternative sigma factor RpoN in PAO1 Free

Abstract

A variety of soil-dwelling bacteria produce polyhydroxybutyrate (PHB), which serves as a source of energy and carbon under nutrient deprivation. Bacteria belonging to the genus do not generally produce PHB but are capable of using the PHB degradation product ()-3-hydroxybutyrate [()-3-HB] as a growth substrate. Essential to this utilization is the NAD-dependent dehydrogenase BdhA that converts ()-3-HB into acetoacetate, a molecule that readily enters central metabolism. Apart from the numerous studies that had focused on the biochemical characterization of BdhA, there was nothing known about the assimilation of ()-3-HB in , including the genetic regulation of expression. This study aimed to define the regulatory factors that govern or dictate the expression of the gene and ()-3-HB assimilation in PAO1. Importantly, expression of the gene was found to be specifically induced by ()-3-HB in a manner dependent on the alternative sigma factor RpoN and the enhancer-binding protein PA2005.This mode of regulation was essential for the utilization of ()-3-HB as a sole source of energy and carbon. However, non-induced levels of expression were sufficient for PAO1 to grow on ( ± )-1,3-butanediol, which is catabolized through an ()-3-HB intermediate. Because this is, we believe, the first report of an enhancer-binding protein that responds to ()-3-HB, PA2005 was named HbcR for ()-3-ydroxyutyrate atabolism egulator.

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© 2015 The Authors
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2015-11-01
2024-12-04
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/content/journal/micro/10.1099/mic.0.000163
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The metabolism of (R)-3-hydroxybutyrate is regulated by the enhancer-binding protein PA2005 and the alternative sigma factor RpoN in Pseudomonas aeruginosa PAO1
Microbiology 161, 2232 (2015); https://doi.org/10.1099/mic.0.000163
/content/journal/micro/10.1099/mic.0.000163
/content/journal/micro/10.1099/mic.0.000163
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