1887

Abstract

is a pleiomorphic fungus that forms mixed species biofilms with , an early colonizer of oral cavity surfaces. Activation of quorum sensing (QS; intercellular signalling) promotes monospecies biofilm development by these micro-organisms, but the role of QS in mixed species communities is not understood. The genes in encode a sensor–regulator system (ComDE), which is activated by the gene product (CSP, competence stimulating peptide) and modulates expression of QS-regulated genes. Dual species biofilms of Δ or Δ mutants with showed increased biomass compared to biofilms of DL1 wild-type with . The Δ mutant dual species biofilms in particular contained more extracellular DNA (eDNA), and could be dispersed with DNase I or protease treatment. Exogenous CSP complemented the Δ transformation deficiency, as well as the Δ- biofilm phenotype. Purified CSP did not affect hyphal filament formation but inhibited monospecies biofilm formation by . The results suggest that the QS-system modulates the production of eDNA and the incorporation of into dual species biofilms.

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2015-02-01
2020-09-27
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