The induction of the heat shock response in subsp. strain MG1363 was analysed at the RNA level using a novel RNA isolation procedure to prevent degradation. Cloning of the and homologues was carried out. Northern blot analysis showed a similar induction pattern for and after transfer from 30 C to 43 C when MG1363 was grown in defined medium. The gene showed a 100-fold induction level 15 min after temperature shifting. Induction of the first two genes in the operon, and resembled the pattern observed for the above genes, although maximum induction was observed earlier for and Novel transcript sizes were detected in heat-shocked cells. The induction kinetics observed for suggested a different regulation for this gene. Experimental evidence for a pronounced transcriptional regulation being involved in the heat shock response in MG1363 is presented. A gene located downstream of the operon in strain MG1363, named was shown not to be regulated by heat shock.


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