1887

Abstract

Summary: The gene encodes ribonuclease II (RNase II), one of the two major exonucleases involved in mRNA degradation. In this paper, the transcript is characterized regarding its promoter and terminator regions. The combined results from S1 nuclease protection analysis, DNase I footprinting and gene fusions with have shown that is expressed from two promoters. S1 nuclease protection analysis and DNA footprinting have shown that has two promoters, P1 and P2. Transcriptional and translational reporter fusions, constructed to the gene, revealed that P2, the proximal promoter, is stronger than P1. However, P2 is not transcribed suggesting that an additional factor is required The 3' end of the transcript mapped to a stem-loop structure immediately after the translated region.

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1996-02-01
2021-03-09
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