1887

Abstract

Covalently bound haem proteins and cytochromes were analysed in biovar free-living cells and nitrogen-fixing bacteroids isolated from pea nodules. Increased levels of spectroscopically detectable cytochrome in bacteroids were correlated with the appearance of two proteins of 30000 and 28000 that contained covalently bound haem. Conversely, bacteroids had undetectable levels of a periplasmic cytochrome of 14000 that is normally present in free-living bacteria. Difference spectra confirmed that the terminal oxidases, cytochromes and were absent, and photodissociation spectra revealed novel components that may be due to a bacteroid terminal oxidase.

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1996-01-01
2024-12-08
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References

  1. Appleby C. A. 1969a; Electron transport systems of Rhizobium japonicum. I. Haemoprotein P-450, other CO-reactive pigments, cytochromes and oxidases in bacteroids from N2-fixing root nodules. Biochim Biophys Acta 172:71–87
    [Google Scholar]
  2. Appleby C. A. 1969b; Electron transport systems of Rhizobium japonicum. II Rhizobium haemoglobin cytochromes and oxidases in free-living (cultured) cells. Biochim Biophys Acta 172:88–105
    [Google Scholar]
  3. Appleby C. A. 1984; Leghemoglobin and Rhizobium respiration. Annu Rev Plant Physiol 35:443–478
    [Google Scholar]
  4. Beynon J. L., Beringer J. E., Johnston A. W. B. 1980; Plasmids and host range in Rhizobium leguminosarum and Rhizobium phaseoli. J Gen Microbiol 120:421–429
    [Google Scholar]
  5. Bott M., Ritz D., Hennecke H. 1991; The Bradyrhizobium japonicum cycM gene encodes a membrane-anchored homolog of mitochondrial cytochrome. J Bacteriol 173:6766–6772
    [Google Scholar]
  6. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
    [Google Scholar]
  7. Bradley D. J., Wood E. A., Larkins A. P., Galfre G., Butcher G. W., Brewin N. J. 1988; Isolation of monoclonal antibodies reacting with peribacteroid membranes and other components of pea root nodules containing Rhizobium leguminosarum. Planta 173:149–160
    [Google Scholar]
  8. Brewin N. J., Robertson J. G., Wood E. A., Wells B., Larkins A. P., Galfre G., Butcher G. W. 1985; Monoclonal antibodies to antigens in the peribacteroid membrane from Rhizobium-induced root nodules of pea cross-react with plasma membrane and Golgi bodies. EMBO J 4:605–611
    [Google Scholar]
  9. Downie J. A., Hombrecher G., Ma Q.-S., Knight C. D., Wells B., Johnston A. W. B. 1983; Cloned nodulation genes of Rhizobium leguminosarum determine host-range specificity. Mol & Gen Genet 190:359–365
    [Google Scholar]
  10. Jones C. W., Poole R. K. 1985; The analysis of cytochromes. Methods Microbiol 18:285–328
    [Google Scholar]
  11. Kahn D, Batut J, Daveran M. L., Fourment J. 1993; Structure and regulation of the fixNOQP operon from Rhizobium meliloti. New Horizons in Nitrogen Fixation474 Edited by Palacios R., Mora J., Newton W. E. Dordrecht: Kluwer Academic Publishers;
    [Google Scholar]
  12. Kretovich W. L., Romanov V. I., Korolyov A. V. 1973; Rhizobium leguminosarum cytochromes (Vicia faba). Plant Soil 39:619–634
    [Google Scholar]
  13. Mandon K., Kaminski P. A., Elmerich C. 1994; Functional analysis of the fixNOQP region of Azorhizpbium caulinodans. J Bacteriol 176:2560–2568
    [Google Scholar]
  14. Poole R. K. 1994; Oxygen reactions with bacterial oxidases and globins: binding, reduction and regulation. Antonie Leeuwenhoek 65:289–310
    [Google Scholar]
  15. Poole R. K., Kumar C., Salmon I., Chance B. 1983; The 650 nm chromophore in Escherichia coli is an ‘oxy-’or oxygenated compound, not the oxidized form of cytochrome oxidase d: an hypothesis. J Gen Microbiol 129:1335–1344
    [Google Scholar]
  16. Poole R. K., Salmon I., Chance B. 1994; The high-spin cytochrome o component of the cytochrome bo-type quinol oxidase in membranes from Escherichia coli: formation of the primary oxygenated species at low temperatures is characterized by a slow ‘on’ rate and low dissociation constant. Microbiology 140:1027–1034
    [Google Scholar]
  17. Preisig O., Anthamatten D., Hennecke H. 1993; Genes for a microaerobically induced oxidase complex in Bradyrhizobium japonicum are essential for a nitrogen-fixing endosymbiosis. Proc Natl Acad Sci USA 90:3309–3313
    [Google Scholar]
  18. Schluter A, Patschkowski T, Weidner S, Unden G, Hynes M. F., Priefer U. B. 1993; Functional and regulatory characteristics of FnrN, an oxygen-responsive transcriptional activator in Rhizobium leguminosarum bv. viciae. New Horizons in Nitrogen Fixation493 Edited by Palacios R., Mora J., Newton W. E. Dordrecht: Kluwer Academic Publishers;
    [Google Scholar]
  19. Sherwood M. T. 1970; Improved synthetic medium for the growth of Rhizobium. J Appl Bacteriol 33:708–713
    [Google Scholar]
  20. Thöny-Meyer L, Stax D., Hennecke H. 1989; An unusual gene cluster for the cytochrome bcx complex in Bradyrhizobium japonicum and its requirement for effective root nodule symbiosis. Cell 57:683–697
    [Google Scholar]
  21. Turner S. R., Ireland R., Rawsthorne S. 1992; Purification and primary amino acid sequence of the L subunit of glycine decarboxylase. J Biol Chem 267:7745–7750
    [Google Scholar]
  22. Vargas C., McEwan A. G., Downie J. A. 1993; Detection of f-type cytochromes using enhanced chemiluminescence. Anal Biochem 209:323–326
    [Google Scholar]
  23. Vargas C., Wu G., Davies A. E., Downie J. A. 1994; Identification of a gene encoding a thioredoxin-like product necessary for cytochrome c biosynthesis and symbiotic nitrogen fixation in Rhizobium leguminosarum. J Bacteriol 176:4117–4123
    [Google Scholar]
  24. Williams H. D., Poole R. K. 1987; The cytochromes of Acetobacter pasteurianus NCIB 6428. Evidence of a role for a cytochrome a1-like haemoprotein in electron transfer to cytochrome oxidase. J Gen Microbiol 133:2461–2472
    [Google Scholar]
  25. Wood P. M. 1984; Bacterial proteins with CO-binding b- or c-type haem. Functions and absorption spectroscopy. Biochim Biophys Acta 768:293–317
    [Google Scholar]
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