1887

Abstract

Covalently bound haem proteins and cytochromes were analysed in biovar free-living cells and nitrogen-fixing bacteroids isolated from pea nodules. Increased levels of spectroscopically detectable cytochrome in bacteroids were correlated with the appearance of two proteins of 30000 and 28000 that contained covalently bound haem. Conversely, bacteroids had undetectable levels of a periplasmic cytochrome of 14000 that is normally present in free-living bacteria. Difference spectra confirmed that the terminal oxidases, cytochromes and were absent, and photodissociation spectra revealed novel components that may be due to a bacteroid terminal oxidase.

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1996-01-01
2021-10-25
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