1887

Abstract

SUMMARY

Growing bacteria are sensitive to various -lactam derivatives due to their interference with peptidogly can biosynthesis. At low concentrations, penicillin G (benzylpenicillin) blocks cell division without affecting mass growth rate. The MIC for division of B/r (H266) was found to depend on the growth rate, which was modified by the nutritional conditions. Our hypothesis, that division sensitivity is proportional to the rate of peptidoglycan synthesis for septum formation, as well as to cell circumference, was thus confirmed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-141-5-1081
1995-05-01
2021-05-13
Loading full text...

Full text loading...

/deliver/fulltext/micro/141/5/mic-141-5-1081.html?itemId=/content/journal/micro/10.1099/13500872-141-5-1081&mimeType=html&fmt=ahah

References

  1. Bi E., Lutkenhaus J. 1991; FtsZ ring structure associated with division in Escherichia coli. . Nature 354:161–164
    [Google Scholar]
  2. Boman H.G., Eriksson K.G. 1963; Penicillin induced lysis in Escherichia coli. . J Gen Microbiol 31:339–352
    [Google Scholar]
  3. Botta G.A., Park J.T. 1981; Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongation.. J Bacteriol 145:333–340
    [Google Scholar]
  4. Donachie W.D. 1993; The cell cycle of Escherichia coli. . Annu Rev Microbiol Al199–230
    [Google Scholar]
  5. Fishov I., Zaritsky A., Grover N.B. 1995; On microbial states of growth.. Mol Microbiol 15: (in press)
    [Google Scholar]
  6. Helmstetter C.E., Cooper S., Pierucci O., Revelas L. 1968; The bacterial life sequence.. Cold Spring Harbor Symp Quant Biol 33809–822
    [Google Scholar]
  7. Miller J.H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Mulder E., Woldringh C.L. 1989; Actively replicating nucleoids influence positioning of division sites in Escherichia coli filaments forming cells lacking DNA.. J Bacteriol 171:4303–4314
    [Google Scholar]
  9. Rosenberger R.F., Grover N.B., Zaritsky A., Woldringh C.L. 1978; Surface growth in rod-shaped bacteria.. J Theor Biol 73:711–721
    [Google Scholar]
  10. Spratt B.G. 1975; Distinct penicillin binding proteins involved in the division, elongation, and shape of Escherichia coli K-12.. Proc Natl Acad Sci USA 722999–3003
    [Google Scholar]
  11. Spratt B.G. 1977; Properties of the penicillin-binding proteins of Escherichia coli K-12.. Eur J Biochem 72:341–352
    [Google Scholar]
  12. Spratt B.G. 1983; Penicillin-binding proteins and the future of β- lactam antibiotics.. J Gen Microbiol 129:1247–1260
    [Google Scholar]
  13. Tuomanen E., Cozens R., Tosch W., Zak O., Tomasz A. 1986; The rate of killing of Escherichia coli by β-lactam antibiotics is strictly proportional to the rate of bacterial growth.. T Gen Microbiol 132:1297–1304
    [Google Scholar]
  14. Waxman D.J., Strominger J.L. 1983; Penicillin-binding proteins and the mechanism of action of β-lactam antibiotics.. Annu Rev Biochem 52:825–869
    [Google Scholar]
  15. Wientjes F.B., Nanninga N. 1991; On the role of the high molecular weight penicillin-binding proteins in the cell cycle of Escherichia coli. . Res Microbiol 142:333–344
    [Google Scholar]
  16. Woldringh C.L., de Jong M.A., van den Berg W., Koppes L. 1977; Morphological analysis of the division cycle of two substrains during slow growth.. J Bacteriol 131:270–279
    [Google Scholar]
  17. Woldringh C.L, Mulder E., Valkenburg J.A.C., Wientjes F.B., Zaritsky A., Nanninga N. 1990; Role of the nucleoid in the toporegulation of division.. Res Microbiol 141:39–49
    [Google Scholar]
  18. Woldringh C.L, Huls P.G., Vischer N.O. 1993; Volume growth of daughter and parent cells during the cell cycle of Saccharomyces cerevisiae a/α as determined by image cytometry.. J Bacteriol 175:3174–3181
    [Google Scholar]
  19. Zaritsky A., Woldringh C.L. 1979; Constant peptidoglycan density in the sacculus of Escherichia coli B/r growing at different rates.. FEBS Lett 98:29–32
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-141-5-1081
Loading
/content/journal/micro/10.1099/13500872-141-5-1081
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error