Summary: A complete isoleucyl-tRNA synthetase gene () of was isolated from a TGH9011 genomic DNA library constructed in pBluescript. The complete coding sequence, flanking regions and transcription start point were determined. The deduced isoleucyl-tRNA synthetase (lleRS) had 917 amino acids with a molecular mass of 105399 Da, which was consistent with the observed size of 105 kDa in maxicells. The gene was mapped onto the physical map of the genome. Alignment of the lleRS sequence with six other bacterial lleRS sequences and two lower eukaryotic lleRS sequences identified seven conserved motifs, including the two signature sequences, HIGH and KMSKS, of class I aminoacyl-tRNA synthetases.


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