1887

Abstract

Summary: The activities of the enzymes involved in ammonium assimilation and glutamate biosynthesis were determined in wild-type and NADP-glutamate dehydrogenase (GDH) null mutant strains of The specific NADP-GDH activity from was fivefold lower than that found in . The glutamine synthetase (GS) and glutamate synthase (GOGAT) activities were similar to those reported in . The NADP-GDH null mutant was obtained by transforming the strain MD2/1 with a linearized integrative yeast vector harbouring a 390 bp fragment of the NADP-GDH structural gene. This mutant grew as well as the parent strain on ammonium, but showed GS and GOGAT activities higher that those found in the wild-type strain, implying that the GS-GOGAT pathway could play a leading role in glutamate biosynthesis in . Southern blotting analysis of chromosomes separated by contour-clamped homogeneous electric field electrophoresis, indicated that the NADP-GDH structural gene is localized on chromosome VI.

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1995-10-01
2021-10-17
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References

  1. Barel I., MacDonald D.W. 1993; Enzyme defects in glutamate- requiring strains of Schizosaccharomyces pombe.. FEMS Microbiol Lett 113:267–272
    [Google Scholar]
  2. Bravo A., Mora J. 1988; Ammonium assimilation in Rhizobium phaseoli by the glutamine synthetase-glutamate synthase pathway.. J Bacteriol 170:980–984
    [Google Scholar]
  3. Castaño I., Flores N., Valle F., Covarrubias A.A., Bolivar F. 1992; gltF, a member of the gltBDF operon of Escherichia coli, is involved in nitrogen-regulated gene expression.. Mol Microbiol 6:2733–2741
    [Google Scholar]
  4. Cogoni C., Valenzuela L., Gonzáiez-Halphen D., Olivera H., Macino G., Ballario P., Gonzáiez A. 1995; Saccharomyces cerevisiae has a single glutamate synthase gene coding for a plant-like high-molecular-weight polypeptide.. J Bacteriol 177:792–798
    [Google Scholar]
  5. Doherty D. 1970; L-Glutamate dehydrogenases (yeast).. Methods Enzymol 17:850–856
    [Google Scholar]
  6. Ferguson A.R., Sims A.P. 1974; The regulation of glutamine metabolism in Candida utilis: the role of glutamine in the control of glutamine synthetase.. J Gen Microbiol 80:159–171
    [Google Scholar]
  7. Folch J.L, Antaramián A., Rodriguíz L., Bravo A., Brunner A., González A. 1989; Isolation and characterization of a Saccharomyces cerevisiae mutant with impaired glutamate synthase activity.. J Bacteriol 171:6776–6781
    [Google Scholar]
  8. Gonzalez A., Deivila G., Calva E. 1985a; Cloning of a DNA sequence that complements glutamine auxotrophy in Saccharomyces cerevisiae.. Gene 36:123–129
    [Google Scholar]
  9. Gonzalez A., Rodriguez L., Olivera H., Soberón M. 1985b; NADP-dependent glutamate dehydrogenase activity is impaired in mutants of Saccharomyces cerevisiae that lack aconitase.. J Gen Microbiol 131:2565–2571
    [Google Scholar]
  10. Helling R.B. 1994; Why does Escherichia coli have two primary pathways for synthesis of glutamate?. J Bacteriol 176:4664–4668
    [Google Scholar]
  11. Hendriks L., Goris A., Van de Peer Y., Neefs Y.M., Vancanneyt M., Kersters K., Berny J.F., Hennebert G.L., De Waechter R. 1992; Phylogenetic relationships among ascomycetes and ascomycetes-like yeasts as deduced from small ribosomal subunit RNA sequences.. Syst Appl Microbiol 15:98–104
    [Google Scholar]
  12. Holmes A.R., Collings A., Farnden K.J.F., Sheperd M.G. 1989; Ammonium assimilation by Candida albicans and other yeasts: evidence for activity of glutamate synthase.. J Gen Microbiol 135:1423–1430
    [Google Scholar]
  13. Holzer H., Schneider S. 1957; Anreicherung und Trennung einer DPN-spezifischen und einer TPN-spezifischen Glutaminosaure Dehydrogenase aus Hefe.. Biochem Z 329:361–367
    [Google Scholar]
  14. Hummelt G., Mora J. 1980; Regulation and function of glutamate synthase in Neurospora crassa.. Biochem Biophys Res Commun 96:1688–1694
    [Google Scholar]
  15. Lloyd A.T., Sharp P.M. 1993; Synonymous codon usage in Kluyveromyces lactis.. Yeast 9:1219–1228
    [Google Scholar]
  16. Lomnitz A., Calderñn J., Hernández G., Mora J. 1987; Functional analysis of ammonium assimilation enzymes in Neurospora crassa.. J Gen Microbiol 133:2333–2340
    [Google Scholar]
  17. Marqués S., Florencio F.J., Candau P. 1992; Purification and characterization of the ferredoxin-glutamate synthase from the unicellular cyanobacterium Synechococcus sp. PCC 6301.. Eur T Biochem 206:69–77
    [Google Scholar]
  18. Meers J.L., Tempest D.W., Brown C.M. 1970; Glutamine(amide):2-oxoglutarate amino transferase oxido-reductase (NADP), an enzyme involved in the synthesis of glutamate by some bacteria.. J Gen Microbiol 64:187–194
    [Google Scholar]
  19. Miflin B.J., Lea P.J., Wallsgrove R.M. 1980; The role of glutamine in ammonium assimilation and reassimilation in plants.. In Glutamine: Metabolism, Enzymology and Regulation pp. 213–234 Mora J., Palacios R. Edited by NY: Academic Press;
    [Google Scholar]
  20. Miller S.M., Magasanik B. 1990; Role of NAD-linked glutamate dehydrogenase in nitrogen metabolism in Saccharomyces cerevisiae.. J Bacteriol 172:4927–4935
    [Google Scholar]
  21. Miranda M., Ramírez J., Guevara S., Ongay-Larios L., Peña A., Coria R. 1995; Nucleotide sequence and chromosomal localization of the gene encoding the old yellow enzyme from Kluyveromyces lactis.. Yeast 11:459–465
    [Google Scholar]
  22. Moye W.S., Amuro N., Rao J.K.M., Zalkin H. 1985; Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase.. J Biol Chem 260:8502–8508
    [Google Scholar]
  23. Nagasu T., Hall B. 1985; Nucleotide sequence of the GDH gene coding for the NADP-specific glutamate dehydrogenase of Saccharomyces cerevisiae.. Gene 37:247–253
    [Google Scholar]
  24. Roon R.J., Even H.L., Larimore F. 1974; Glutamate synthase: properties of the reduced nicotinamide adenine dinucleotide- dependent enzyme from Saccharomyces cerevisiae.. J Bacteriol 118:89–95
    [Google Scholar]
  25. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Senior P.J. 1975; Regulation of nitrogen metabolism in Escherichia coli and Klebsiella aerogenes: studies with the continuous- culture technique.. J Bacteriol 123:407–418
    [Google Scholar]
  27. Sor F., Fukuhara F. 1989; Analysis of chromosomal DNA patterns of the genus Kluyveromyces.. Yeast 5:1–10
    [Google Scholar]
  28. Tempest D.W., Meers J.L., Brown C.M. 1970; Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route.. Biochem J 117:405–407
    [Google Scholar]
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