This paper is concerned with the application of Metabolic Control Analysis to chemostat experiments. It deals with the peculiar problems which are presented when using a chemostat for such purposes. The paper shows how to apply a top-down approach to quantifying control in the chemostat exerted by both the ‘physical’ components (e.g. dilution rate) and the biological components. The analysis is considered at a number of levels, depending on the amount of information on the components of metabolism either known or accessible experimentally. A method is also presented for using the information obtained by this analysis to obtain a ‘virtual’ control coefficient, which is defined as a control coefficient which would be obtained if it were possible to clamp all metabolites ‘external’ to the micro-organism, including biomass, at their steady-state levels. This allows, amongst other things, control coefficients with respect to growth rate to be determined under chemostat conditions.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error