We have cloned a gene, which encodes a cyclic nucleotide phosphodiesterase (PDEase), by complementation in a PDEase-deficient mutant. The deduced amino acid sequence is similar to that of the low-affinity PDEase of (PDE1) and the cyclic nucleotide PDEase (PD) of Biochemical analysis of recombinant protein produced in indicated that the enzyme behaves as a PDE1 homologue: it hydrolyses both cAMP ( = 0.49 mM) and cGMP ( = 0.25 mM), does not require divalent cations for maximal activity and is only moderately inhibited by millimolar concentrations of standard PDEase inhibitors. Based on these data, we designate the we have cloned, Low-stringency genomic Southern blots showed cross-hybridization between and DNA from , but not with DNA from or several closely related species.


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