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Volume 140, Issue 7

A cyclic nucleotide phosphodiesterase: cloning and expression in and biochemical characterization of the recombinant enzyme Free

Abstract

We have cloned a gene, which encodes a cyclic nucleotide phosphodiesterase (PDEase), by complementation in a PDEase-deficient mutant. The deduced amino acid sequence is similar to that of the low-affinity PDEase of (PDE1) and the cyclic nucleotide PDEase (PD) of . Biochemical analysis of recombinant protein produced in indicated that the enzyme behaves as a PDE1 homologue: it hydrolyses both cAMP ( = 0·49 mM) and cGMP ( = 0·25 mM), does not require divalent cations for maximal activity and is only moderately inhibited by millimolar concentrations of standard PDEase inhibitors. Based on these data, we designate the we have cloned, . Low-stringency genomic Southern blots showed cross-hybridization between and DNA from , but not with DNA from or several closely related species.

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Keyword(s): Candida albicans , cyclic nucleotide phosphodiesterase and morphogenesis
© Society for General Microbiology 1994
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1994-07-01
2024-04-26
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A Candida albicans cyclic nucleotide phosphodiesterase: cloning and expression in Saccharomyces cerevisiae and biochemical characterization of the recombinant enzyme
Microbiology 140, 1533 (1994); https://doi.org/10.1099/13500872-140-7-1533
/content/journal/micro/10.1099/13500872-140-7-1533
/content/journal/micro/10.1099/13500872-140-7-1533
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