1887

Abstract

-like organisms were isolated from the liver, duodenum and caecum of broiler and layer chickens, and from humans with gastroenteritis. They formed a unique DNA homology group and a polyphasic taxonomic analysis was made of 16 strains. Analysis of the nucleotide sequence of the 16S rRNA gene from seven of the strains identified them as belonging to a single species, within the genus This conclusion was supported by the studies of relative DNA homology and of total protein electrophoretic patterns. The new species could be biochemically differentiated from other helicobacters and its ultrastructure in the electron microscope was typical of the genus except that the flagellum was not sheathed. We propose the name sp. nov. for this group. Like or it represents another non-gastric urease-negative species colonizing the lower bowel. Its isolation from the livers of chickens with vibrionic hepatitis is significant. We describe a species-specific PCR assay for sp. nov. which will facilitate its identification and further studies of its epidemiology.

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1994-12-01
2021-10-23
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References

  1. Blaser M.J. Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis 1990; 161:626–633
    [Google Scholar]
  2. Blaser J. Helicobacter pylori: microbiology of a ‘slow’ bacterial infection. Trends Microbiol 1993; 1:255–260
    [Google Scholar]
  3. Boivin M.F., Morris V.L., Lee-Chan E.C.M., Murray R.G.E. Deoxyribonucleic acid relatedness between selected members of the genus Aquaspirillum by slot blot hybridization: AquaspiriIlium serpens (Mueller 1786) Hylemon, Wells, Krieg and Jannasch 1973 emended to include Aquaspirillum bengal as a subjective synonym. Int J Syst Bacteriol 1985; 35:512–517
    [Google Scholar]
  4. Burnens A.P., Nicolet J. Detection of Campylobacter upsaliensis in diarrheic dogs and cats, using a selective medium with cefoperazone. Am J Vet Res 1992; 53:48–51
    [Google Scholar]
  5. Costas M. Classification, identification and typing of bacteria by the analysis of their one-dimensional polyacrylamide gel electrophoresis protein patterns. In Advances in Electrophoresis 1992 Edited by Chambrach A., Dunn M.J., Radola B.J. Weinheim : VCH Verlagsgesellschaft; pp 351–408
    [Google Scholar]
  6. Dewhirst F.E., Seymour C., Fraser G.J., Paster B.J., Fox J.G. Phylogeny of Helicobacter isolates from bird and swine feces and description of Helicobacter pametensis sp. nov. Int J Syst Bacteriol 1994; 44:553–560
    [Google Scholar]
  7. Eaton K.A., Dewhirst F.E., Radin M.J., Fox J.G., Paster B.J., Krakowka S., Morgan D.R. Helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. Int J Syst Bacteriol 1993; 43:99–106
    [Google Scholar]
  8. Embley T.M. The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 1991; 13:171–174
    [Google Scholar]
  9. Fox J.G., Dewhirst F.E., Tully J.G., Paster B.J., Yan L., Taylor N.S., Collins M.J. Jr, Gorelick P.L., Ward J.M. Helicobacter hepaticus sp nov, a microaerophilic bacterium isolated from the livers and intestinal mucosal scrapings from mice. J Clin 1994; Microbiol32:1238–1245
    [Google Scholar]
  10. Han Y.-H., Smibert R.M., Krieg N.R. Occurrence of sheathed flagella in Campylobacter cinaedi and Campylobacter fennelliae. Int J Syst Bacteriol 1989; 39:488–490
    [Google Scholar]
  11. Holmes B., On S.L.W., Costas M., Ganner M. Some new applications of probabilistic identification. In Proceedings of the Conference on Taxonomy and Automated Identification of Bacteria 1992 Edited by Schindler J. Prague: Czechoslovak Society for Microbiology; pp 6–8
    [Google Scholar]
  12. Jukes T.H., Cantor C.R. Evolution of protein molecules. In Mammalian Protein Metabolism 1969 Edited by Munro H.N. New York: Academic Press; pp 21–132
    [Google Scholar]
  13. Kusukawa N., Uemori T., Asada K., Kato I. Rapid and reliable protocol for direct sequencing of material amplified by the polymerase chain reaction. Biotechniques 1990; 9:66–72
    [Google Scholar]
  14. Lee A.S., G. & McGee J.O. The signal intensity on Southern blots developed by nonisotopic methods is linear with time and quantity of DNA. Nucleic Acids Res 1989; 17:2364
    [Google Scholar]
  15. Lior H., Patel A. Improved toludine blue-DNA agar for detection of DNA hydrolysis by Campylobacters. J Clin Microbiol 1987; 25:2030–2031
    [Google Scholar]
  16. Mishu B., Patton C.M., Tauxe R.V. Clinical and epidemiological features of non-jejuni, non-coli Campylobacter species. In Campylobacter jejuni: Current Status and Future Trends 1993 Edited by Nachamkin I., Blaser M.J., Tompkins L.S. Washington, DC: American Society for Microbiology; pp 31–41
    [Google Scholar]
  17. On S.L.W., Holmes B. Effect of inoculum size on the phenotypic characterization of Campylobacter spp. J Clin Microbiol 1991a; 29:923–926
    [Google Scholar]
  18. On S.L.W., Holmes B. Reproducibility of tolerance tests that are useful in the identification of campylobacteria. J Clin Microbiol 1991b; 29:1785–1788
    [Google Scholar]
  19. On S.L.W., Holmes B. Assessment of enzyme detection tests useful in identification of campylobacteria. J Clin Microbiol 1992; 30:746–749
    [Google Scholar]
  20. Owen R.J., Pitcher D.G. Chemical methods for estimating DNA base compositions and levels of DNA-DNA hybridization. In Chemical Methods in Bacterial Systematics 1985 Edited by Goodfellow M., Minnikin D.E. London: Academic Press; pp 67–93
    [Google Scholar]
  21. Paster B.J., Dewhirst F.E. Phylogeny of Campylobacters, Wolinellas, Bacteroidesgracilis, and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int J Syst Bacteriol 1988; 38:56–62
    [Google Scholar]
  22. Saitou N., Nei M. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
    [Google Scholar]
  23. Seymour C., Lewis R.J., Kim M., Gagnon D.F., Fox J.G., Dewhirst F.E., Paster B.J. Isolation of Helicobacter strains from wild bird and swine feces. Appl Environ Microbiol 1994; 60:1025–1028
    [Google Scholar]
  24. Stanley J., Burnens A.P., Linton D., On S.L.W., Costas M., Owen R.J. Campylobacter helveticus sp. nov., a new thermophilic species from domestic animals: characterization and cloning of a species-specific DNA probe. J Gen Microbiol 1992; 138:2293–2303
    [Google Scholar]
  25. Stanley J., Linton D., Burnens A.P., Dewhirst F.E., Owen R.J., Porter A., On S.L.W., Costas M. Helicobacter canis sp. nov., a new species from dogs: an integrated study of phenotype and genotype. J Gen Microbiol 1993; 139:2495–2504
    [Google Scholar]
  26. Stern N.J. Reservoirs for Campylobacter jejuni and approaches for intervention in poultry. In Campylobacter jejuni: Current Status and Future Trends 1993 Edited by Nachamkin I., Blaser M.J., Tompkins L.S. Washington, DC: American Society for Microbiology; pp 49–60
    [Google Scholar]
  27. Totten P.A., Fennell C.L., Tenover F.C., Wezenberg J.M., Perine P.L., Stamm W.E., Holmes K.K. Campylobacter cinaedi (sp. nov.) and Campylobacter fennelliae (sp. nov.): two new Campylobacter species associated with enteric disease in homosexual men. J Infect Dis 1985; 151:131–139
    [Google Scholar]
  28. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol 1991; 41:88–103
    [Google Scholar]
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